One-Step Lumitein™ UV Protein Gel Stain
A ready-to-use luminescent protein gel stain optimized for UV-based fluorescence imaging systems. Non-toxic, with no fixation required.
One-Step Lumitein™ UV Protein Gel Stain is a ready-to-use luminescent red protein gel stain optimized for use on UV transilluminators. With no fixation or wash steps required, One-Step Lumitein™ UV is a great choice for convenience and safety. One-Step Lumitein™ UV can detect as little as approximately 1-10 ng of protein per band.
- One-step staining procedure, no fixation required
- Non-toxic formulation is safe for easy disposal
- Sensitive protein detection down to 1-10 ng
- Optimized for use with a UV transilluminator
- 1 L and 4 L sizes
- Store at 4°C
Fast and simple procedure
One-Step Lumitein™ UV gel staining requires only a single 5-30 minute staining step without fixation. Destaining is optional. One-Step Lumitein™ UV can detect as little as approximately 1-10 ng of protein per band depending on the staining method used, although staining intensity varies between proteins. Staining is fully compatible with mass spectrometry and Edman-based sequencing. Gels stained with One-Step Lumitein™ UV Protein Gel Stain can be visualized with a variety of different UV-based fluorescence imaging systems.
The results obtained with One-Step Lumitein™ UV are comparable to those with Oriole™ Fluorescent Gel Stain; however, One-Step Lumitein™ UV has a more convenient protocol, does not contain hazardous solvents, and does not cause gel shrinkage. Furthermore, One-Step Lumitein™ costs significantly less than other fluorescent protein gel stains. One-Step Lumitein™ UV Protein Gel Stain is available in 1 liter bottle or 4 liter Cubitainer® sizes.
One-Step Lumitein™ UV is non-toxic
One-Step Lumitein™ UV Protein Gel Stain offers safer handling and ease of disposal, because it is an aqueous-based solution that does not contain hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.
Please visit our Protein Gel Stains technology page to learn about our other safe and sensitive protein gel stains such as One-Step Lumitein™ and One-Step Blue™, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue staining.
|Product||Replacement for||Catalog number||Unit size||Product protocol||Safety report|
|One-Step Blue®||Coomassie Brilliant Blue stains||21003-1L||1L||PI-21003||One-Step Gel Stains Safety Report|
|One-Step Lumitein™||SYPRO® Ruby Protein Gel Stain||21004-1L||1L||PI-21004|
|One-Step Lumitein™ UV||Oriole™ and Flamingo™ Protein Gel Stains||21005-1L||1L||PI-21005|
Nature Biotechnology (2018), doi:10.1038/nbt.4173