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One-Step Lumitein™ UV Protein Gel Stain

A ready-to-use luminescent protein gel stain optimized for UV-based fluorescence imaging systems. Non-toxic, with no fixation required.

Product Attributes

Excitation/Emission

288/603 nm

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1 L
4 L
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Product Description

One-Step Lumitein™ UV Protein Gel Stain is a ready-to-use luminescent red protein gel stain optimized for use on UV transilluminators. With no fixation or wash steps required, One-Step Lumitein™ UV is a great choice for convenience and safety. One-Step Lumitein™ UV can detect as little as approximately 1-10 ng of protein per band.

  • One-step staining procedure, no fixation required
  • Non-toxic formulation is safe for easy disposal
  • Sensitive protein detection down to 1-10 ng
  • Optimized for use with a UV transilluminator
  • 1 L and 4 L sizes
  • Store at 4°C

Fast and simple procedure

One-Step Lumitein™ UV gel staining requires only a single 5-30 minute staining step without fixation. Destaining is optional. One-Step Lumitein™ UV can detect as little as approximately 1-10 ng of protein per band depending on the staining method used, although staining intensity varies between proteins. Staining is fully compatible with mass spectrometry and Edman-based sequencing. Gels stained with One-Step Lumitein™ UV Protein Gel Stain can be visualized with a variety of different UV-based fluorescence imaging systems.

The results obtained with One-Step Lumitein™ UV are comparable to those with Oriole™ Fluorescent Gel Stain; however, One-Step Lumitein™ UV has a more convenient protocol, does not contain hazardous solvents, and does not cause gel shrinkage. Furthermore, One-Step Lumitein™ costs significantly less than other fluorescent protein gel stains. One-Step Lumitein™ UV Protein Gel Stain is available in 1 liter bottle or 4 liter Cubitainer® sizes.

One-Step Lumitein™ UV is non-toxic

One-Step Lumitein™ UV Protein Gel Stain offers safer handling and ease of disposal, because it is an aqueous-based solution that does not contain hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Red fluorescent One-Step Lumitein™ UV Protein Gel Stain is designed for imaging using a UV transilluminator. It is an alternative to Oriole™ Fluorescent Gel Stain, but does not contain hazardous solvents and does not cause gel shrinkage.

Please visit our Protein Gel Stains technology page to learn about our other safe and sensitive protein gel stains such as One-Step Lumitein™ and One-Step Blue™, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue staining.

ProductReplacement forCatalog numberUnit sizeProduct protocolSafety report
One-Step Blue®
Coomassie stain21003-1L1LPI-21003
One-Step Gel Stains Safety Report
One-Step Lumitein™SYPRO® Ruby Protein Gel Stain21004-1L1LPI-21004
21004-4L4L
One-Step Lumitein™ UVOriole™ and Flamingo™ Protein Gel Stains21005-1L1LPI-21005
21005-4L4L
One-Step Blue is a registered trademark of Biotium. Lumitein and its related technologies are covered by US and international patents. SYPRO is a registered trademark of Molecular Probes, Inc. Oriole and Flamingo are trademarks of Bio-Rad Laboratories. Cubitainer is a registered trademark of Hedwin Corporation.

References

Nature Biotechnology (2018), doi:10.1038/nbt.4173

 

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