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One-Step Blue™ & One-Step Lumitein™ Protein Gel Stains


Our One-Step stains are low-cost, quick, convenient and easy-to-use protein gel stains. Staining is quick, 5-60 min in a single step without fixation or washing. These stains have non-toxic formulations for safer handling and easy disposal. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step. The One-Step stains are compatible with Edman-based sequencing and with mass spectrometry.

100% H2O-based formulation

NO stinky acetic acid

NO toxic methanol

NO microwaving

NO washing

NO fixing

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One-Step Blue™ is an alternative to tedious Coomassie staining, and can be imaged visually or using a near-infrared fluorescence imager.
One-Step-Lumitein gelRed fluorescent One-Step Lumitein™ is an alternative to time-consuming and expensive SYPRO® Ruby protein gel stain, and it can be imaged on a UV box or fluorescence gel scanner.

Lumitein vs Oriole

Red fluorescent One-Step Lumitein™ UV Protein Gel Stain is designed for imaging using a UV transilluminator. It is an alternative to Oriole™ Fluorescent Gel Stain, but does not contain hazardous solvents and does not cause gel shrinkage.

Ordering information

ProductReplacement forCatalog numberUnit size
One-Step Blue™Coomassie stain21003-1L1L
One-Step Lumitein™SYPRO® Ruby Protein Gel Stain21004-1L1L
One-Step Lumitein™ UVOriole™ Protein Gel Stain21005-1L1L

Lumitein and its related technologies are covered by US and international patents.
SYPRO is a registered trademark of Molecular Probes, Inc.
Oriole is a trademark of Bio-Rad Laboratories.