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One-Step Blue™, One-Step Lumitein™ & One-Step Lumitein UV™

One-Step Protein Gel Stains
Low-cost, rapid, non-toxic protein gel stains
One-Step Blue™
Alternative to tedious Coomassie staining
One Step Lumitein & One Step Lumitein UV
Replacements for costly and time consuming SYPRO Ruby and Oriole stains

Our One-Step protein gel stains are low-cost, quick, convenient and easy-to-use for protein gel staining. Staining is quick: 5 to 60 min in a single step without fixation or washing. These stains have non-toxic formulations for safer handling and easy disposal. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

The One-Step stains are compatible with Edman-based sequencing and with mass spectrometry.


100% H2O-based formulation

NO stinky acetic acid

NO toxic methanol

NO microwaving

NO washing

NO fixing

One-Step Blue™ is an alternative to tedious Coomassie staining, and can be imaged visually or using a near-infrared fluorescence imager.
Red fluorescent One-Step Lumitein™ is an alternative to time-consuming and expensive SYPRO® Ruby protein gel stain, and it can be imaged on a UV box or laser gel scanner
One-Step Lumitein™ UV Gel Stain is an alternative to Oriole™ Gel Stain for UV transilluminator. It does not contain hazardous solvents or shrink gels.

Product Information

Product Replacement for Catalog number Unit size Product protocol Safety report
One-Step Blue™ Coomassie stain 21003-1L 1L PI-21003
One-Step Lumitein™ SYPRO® Ruby Protein Gel Stain 21004-1L 1L PI-21004
21004-4L 4L
One-Step Lumitein™ UV Oriole™ Protein Gel Stain 21005-1L 1L PI-21005
21005-4L 4L

Lumitein and its related technologies are covered by US and international patents.
SYPRO is a registered trademark of Molecular Probes, Inc.
Oriole is a trademark of Bio-Rad Laboratories.

One-Step Blue Protein Gel Stain

From: $89 Sizes: 1 LCatalog #: 21003-1L

One-Step Lumitein Protein Gel Stain

From: $115 Sizes: 1 L, 4 LCatalog #: 21004-1L, - 21004-4LView allHide

One-Step Lumitein UV Protein Gel Stain

From: $155 Sizes: 1 L, 4 LCatalog #: 21005-1L, - 21005-4LView allHide