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One-Step Blue® Protein Gel Stain

A visible, blue protein gel staining solution. Fast protein staining in a single step without fixation or washing.

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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1 L
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Product Description

One-Step Blue® Protein Gel Stain is a ready-to-use visible blue protein gel staining solution. With no fixation or wash steps required, One-Step Blue® is a great choice for convenience and safety. One-Step Blue® can detect as little as approximately 10-20 ng of protein per band.

  • One-step staining procedure, no fixation required
  • Non-toxic formulation is safe for easy disposal
  • Sensitive protein detection down to 10-20 ng
  • Visualize blue bands, or image with a near-infrared fluorescence imager
  • 1 L size
  • Store at 4°C

Fast and simple procedure

One-Step Blue® produces fast (5-60 min) protein staining in a single step without fixation or washing. Destaining is optional. One-Step Blue® has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins. The staining is fully compatible with mass spectrometry and Edman-based sequencing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence.

Results are clear. One-Step Blue® is a superior alternative to Coomassie and other colorimetric protein gel stains.

In a comparison experiment with Coomassie Brilliant Blue R-250 and Simply Blue™ SafeStain, One-Step Blue® demonstrated much clearer staining after 1 hour of destaining in water compared to other stains.

Unstained protein ladder was loaded on a 4-12% Bis-Tris gel and stained for 1 hour with Coomassie Brilliant Blue R-250 (CBB), Simply Blue™ SafeStain (SB), and One-Step Blue® (OSB) following SDS-PAGE. Gels stained with Simply Blue™ SafeStain, and One-Step Blue® were destained in water for 1 hour. The gel stained with CBB was destained in 5% MeOH, and 7.5% acetic acid for 1 hour.

One-Step Blue® is non-toxic

One-Step Blue® offers safer handling and disposal compared to Coomassie staining because it is entirely aqueous-based, without hazardous methanol or acetic acid.  One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

One-Step Blue® is an alternative to tedious Coomassie staining, and can be imaged visually (left) or using a near-infrared fluorescence imager (right).

Please visit our Protein Gel Stains technology page to learn about our other safe and sensitive protein gel stains such as One-Step Lumitein™ and One-Step Lumitein UV which are rapid, easy-to-use, and non-toxic alternative to SYPRO® Ruby and Oriole™ Protein Gel Stains .

ProductReplacement forCatalog numberUnit sizeProduct protocolSafety report
One-Step Blue®
Coomassie stain21003-1L1LPI-21003
One-Step Gel Stains Safety Report
One-Step Lumitein™SYPRO® Ruby Protein Gel Stain21004-1L1LPI-21004
One-Step Lumitein™ UVOriole™ and Flamingo™ Protein Gel Stains21005-1L1LPI-21005
One-Step Blue is a registered trademark of Biotium. Lumitein and its related technologies are covered by US and international patents. SYPRO is a registered trademark of Molecular Probes, Inc. Oriole and Flamingo are trademarks of Bio-Rad Laboratories. Cubitainer is a registered trademark of Hedwin Corporation. SimplyBlue is a trademark of Thermo Fisher Scientific.

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