Coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced.
Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature or with an ice pack. These products may thaw without affecting product performance. When you receive the product, place it under the recommended storage conditions.
Biotium ships all antibodies (primary, secondary and conjugates) at room temperature. We guarantee their quality and performance under these conditions based upon our stability testing. Antibodies were subjected to accelerated stability testing by storing them at various temperatures (4°C, room temperature, or 37°C) for 1 week to mimic simulated shipping conditions and tested in immunostaining experiments. All antibodies showed the expected brightness and specificity, even after storage at sub-optimal temperatures.
In line with our goal to be more environmentally friendly by reducing the use of excess packaging, and lowering shipping costs for our customers, products that have passed our stability testing are shipped at room temperature.
Once you have received the antibody vial, please follow the long-term storage instructions on the product information (PI) sheet.
One-Step Blue® Protein Gel Stain is a ready-to-use visible blue protein gel staining solution. With no fixation or wash steps required, One-Step Blue® is a great choice for convenience and safety. One-Step Blue® can detect as little as approximately 10-20 ng of protein per band.
One-step staining procedure, no fixation required
Non-toxic formulation is safe for easy disposal
Sensitive protein detection down to 10-20 ng
Visualize blue bands, or image with a near-infrared fluorescence imager
1 L size
Store at 4°C
Fast and simple procedure
One-Step Blue® produces fast (5-60 min) protein staining in a single step without fixation or washing. Destaining is optional. One-Step Blue® has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins. The staining is fully compatible with mass spectrometry and Edman-based sequencing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence.
Results are clear. One-Step Blue® is a superior alternative to Coomassie and other colorimetric protein gel stains.
In a comparison experiment with Coomassie Brilliant Blue R-250 and Simply Blue™ SafeStain, One-Step Blue® demonstrated much clearer staining after 1 hour of destaining in water compared to other stains.
One-Step Blue® is non-toxic
One-Step Blue® offers safer handling and disposal compared to Coomassie staining because it is entirely aqueous-based, without hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.
One-Step Blue is a registered trademark of Biotium. Lumitein and its related technologies are covered by US and international patents. SYPRO is a registered trademark of Molecular Probes, Inc. Oriole and Flamingo are trademarks of Bio-Rad Laboratories. Cubitainer is a registered trademark of Hedwin Corporation. SimplyBlue is a trademark of Thermo Fisher Scientific.