Support & Resources

+1

Permeabilization and Blocking Buffer (5X)

A concentrated 5X buffer solution for one step permeabilization and blocking for immunofluorescence staining for fluorescence microscopy.

Product Attributes

Storage Conditions

Store at -10 to -35 °C

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Size
Catalog #
price
Qty
100 mL
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Protocols
SDS
Supporting Documents

Product Description

The Permeabilization and Blocking Buffer (5X) is a concentrated stock solution for one step cell permeabilization and blocking. It is designed for use during immunofluorescence staining for fluorescence microscopy.

  • Permeabilizes fixed cells for immunofluorescence staining
  • Blocks non-specific binding of antibodies
  • Can be used to dilute antibodies for staining, and as a wash buffer
  • Contains goat serum, not for use with anti-goat or anti-sheep antibodies
  • 100 mL of 5X solution

Note: this buffer contains goat serum and therefore is not compatible with anti-goat and anti-sheep secondary antibodies. We recommend Permeabilization Buffer (22016) and 10% Fish Gelatin Blocking Agent (22010) for use with anti-goat and anti-sheep secondary antibodies.

 

You may also like…