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Permeabilization and Blocking Buffer (5X)

A concentrated 5X buffer solution for one step permeabilization and blocking for immunofluorescence staining for fluorescence microscopy.

Product Attributes

Storage Conditions

Store at -10 to -35 °C

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Catalog #
100 mL
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Supporting Documents

Product Description

The Permeabilization and Blocking Buffer (5X) is a concentrated stock solution for one step cell permeabilization and blocking. It is designed for use during immunofluorescence staining for fluorescence microscopy.

  • Permeabilizes fixed cells for immunofluorescence staining
  • Blocks non-specific binding of antibodies
  • Can be used to dilute antibodies for staining, and as a wash buffer
  • Contains goat serum, not for use with anti-goat or anti-sheep antibodies
  • 100 mL of 5X solution

Note: this buffer contains goat serum and therefore is not compatible with anti-goat and anti-sheep secondary antibodies. We recommend Permeabilization Buffer (22016) and 10% Fish Gelatin Blocking Agent (22010) for use with anti-goat and anti-sheep secondary antibodies.


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