Ready to use permeabilization buffer for intracellular immunofluorescence staining for microscopy or flow cytometry. Also available in the Flow Cytometry Fixation/Permeabilization Kit (cat. 23006).
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Flow Cytometry Fixation/Permeabilization Kit
Flow Cytometry Fixation/Permeabilization Kit contains optimally formulated buffers for fixation and permeabilization of suspension cells for immunofluorescence staining of intracellular antigens for analysis by flow cytometry. Note: the fixation buffer in this kit is prepared from methanol-stabilized formaldehyde. For methanol-free fixative, see our Paraformaldehyde, 4% in PBS, Ready-to-Use Fixative.
Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry. Also available in the Flow Cytometry Fixation/Permeabilization Kit (cat. 23006). Note: this fixative is prepared with methanol-stabilized formaldehyde. For methanol-free formaldehyde fixative, see our Paraformaldehyde, 4% in PBS, Ready-to-Use Fixative.
Permeabilization and Blocking Buffer (5X)
Concentrated buffer stock solution (5X) for one step permeabilization and blocking for immunofluorescence staining. Note: this buffer contains goat serum and therefore is not compatible with anti-goat and anti-sheep secondary antibodies.
EverBrite™ Hardset Mounting Medium
A revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. EverBrite™ Hardset mounting medium hardens at room temperature completely in 24 hours.
10X Fish Gelatin Blocking Agent
10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.