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Pluronic® F-127

Nonionic detergent useful for solubilizing hydrophobic compounds to facilitate cell loading.

Product Attributes

CAS number

9003-11-6

Applications

Ion indicator accessory reagents

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1 g
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Product Description

Pluronic® F-127 is a nonionic detergent useful for solubilizing hydrophobic dye molecules such as cell-permeable AM esters of Fura-2, Fluo-3, Indo-1, or Rhod-2 calcium indicators to facilitate cell loading. Pluronic® F-127 is usually dissolved in water or DMSO with gentle heating. We also offer ready-to-use solutions of Pluronic® F-127, 20% in DMSO and Pluronic® F-127, 10% in H2O.

  • White solid soluble in water and DMSO
  • Store at room temperature
  • [9003-11-6]

Pluronic F-127 is a registered trade mark of BASF.

References

1) J. Membrane Biol. 19, 1 (1974).
2) Science 233, 886 (1986).
3) J. Biol Chem 262, 12801 (1987).
4) J. Biol. Chem. 265, 19543 (1990).
5) Methods Enzymol. 302, 341 (1999).
6) Methods Enzymol. 307, 441(1999).

 

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    Fluo-4 AM Ester

    Membrane-permeant form of Fluo-4 calcium indicator. Fluo-4 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-4 by endogenous esterases once inside cells.

    Fluo-3, AM Ester

    Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading.

    Rhod-2, AM Ester

    Membrane-permeant form of Rhod-2 calcium indicator that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 is often used as a dispersing agent to facilitate dye loading.

    Fura-2, AM Ester

    Fura-2, AM ester is a ratiometric calcium indicator that is membrane-permeant and can thus be loaded into cells via incubation.

    Indo-1, AM Ester

    Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate cell loading.

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.