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Rhod-590 AM Ester

Membrane-permeant form of Rhod-590 that can be loaded into cells via incubation.

Product Attributes

Applications

Calcium indicators

Cell permeability

Membrane permeant

Indicator type

Non-ratiometric

Excitation/Emission

590/616 nm (no Ca2+); 595/626 nm (high Ca2+) (after hydrolysis)

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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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10 x 50 ug
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Product Description

Membrane-permeant form of Rhod-590 calcium indicator dye that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic® F-127 (cat# 59004) is often used as a dispersing agent to facilitate cell loading. Rhod-590 AM ester itself does not bind Ca2+, but it is readily hydrolyzed to Rhod-590 by endogenous esterases once it enters cells. For more information on rhod-590, see cat# 50026.

  • Dark purple solid soluble in DMSO
  • Store at -20°C and protect from light, especially in solution
  • MW: 1204

 


 
 
BAPTA-based ion indicators like Rhod-590 have been shown to be fixable in situ by EDC/EDAC (cat# 59002). The fixation of indicator dyes is useful for downstream immunofluorescence and IHC studies (Cell Calcium 1997, 21(3), 175).
 

Pluronic is a registered trademark of BASF.
 

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