Rhod-590, Tripotassium Salt
Membrane-impermeant calcium indicator that can be loaded into cells via microinjection or scrape loading. For the membrane-permeable AM ester form of Rhod-590, see 50025.
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Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
Calcium Calibration Buffer Kit
This kit provides buffers for generating a range of accurate calcium concentrations for the calibration of fluorescent Ca2+ indicators.
Probenecid, Sodium Salt, Water Soluble
Probenecid is commonly used to inhibit organic-anion transporters located in the cell membrane. Such transporters can extrude dyes and indicators and thus contribute to low signal or high background in assays due to poor cellular retention of dyes.
Fluo-3, AM Ester
Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading.