Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF® Dye or Biotin Tyramide
Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.
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Product Description
This kit contains reagents for performing tyramide signal amplification (TSA) using goat anti-mouse HRP secondary antibody and fluorescent CF® Dyes or biotin to increase immunofluorescence signal.
Features
- Increase sensitivity by up to 100-fold
- Use lower primary antibody concentrations
- Boost signal in challenging samples, like human FFPE tissues
- Choose from 6 bright & photostable CF® dyes or biotin
Kit Components:
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to conventional avidin-biotin detection. Multiple rounds of tyramide signal amplification can be performed for multiplex detection; to learn more, see our Tech Tip: Multicolor Fluorescence Imaging using Tyramide Amplification Kits. Learn more about tyramide signal amplification.
The HRP goat anti-mouse antibody provided in the kits has been adsorbed against bovine, horse, human, rabbit, and swine serum to minimize cross-reactivity; the antibody is not cross-adsorbed against rat. The kits include your choice of 6 CF® dye tyramides or biotin tyramide. CF® dyes are Biotium’s line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Learn more about CF® dyes.
Also see our Tyramide Amplification Kits with HRP Goat Anti-Rabbit and Tyramide Amplification Kits with HRP Streptavidin.
Each kit contains reagents for staining 50-150 slides; the actual number of samples that can be stained will depend on staining volumes used. Tyramide Amplification Buffer, CF® Dye Tyramides, and other tyramide compounds are also available separately. See our full selection of tyramide products.
Tyramide Signal Amplification Kits
Tyramide Label | Ex/Em | Secondary conjugate | Catalog no. |
---|---|---|---|
CF®488A | 490/515 nm | Goat anti-mouse HRP | 33000 |
Goat anti-rabbit HRP | 33001 | ||
Streptavidin HRP | 33002 | ||
CF®543 | 541/560 nm | Goat anti-mouse HRP | 33003 |
Goat anti-rabbit HRP | 33004 | ||
Streptavidin HRP | 33005 | ||
CF®568 | 562/583 nm | Goat anti-mouse HRP | 33006 |
Goat anti-rabbit HRP | 33007 | ||
Streptavidin HRP | 33008 | ||
CF®594 | 593/614 nm | Goat anti-mouse HRP | 33009 |
Goat anti-rabbit HRP | 33010 | ||
Streptavidin HRP | 33011 | ||
CF®640R | 642/662 nm | Goat anti-mouse HRP | 33012 |
Goat anti-rabbit HRP | 33013 | ||
Streptavidin HRP | 33014 | ||
CF®680R | 680/701 nm | Goat anti-mouse HRP | 33015 |
Goat anti-rabbit HRP | 33016 | ||
Streptavidin HRP | 33017 | ||
Biotin-XX | N/A | Goat anti-mouse HRP | 33018 |
Goat anti-rabbit HRP | 33019 | ||
Streptavidin HRP | 33020 |