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Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF® Dye or Biotin Tyramide

Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

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Product Description

Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.  The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. Multiple rounds of tyramide signal amplification can be performed for multiplex detection; to learn more, see our Tech Tip: Multicolor Fluorescence Imaging using Tyramide Amplification Kits.

Learn more about tyramide signal amplification.

The kit contains HRP goat anti-mouse with a choice of 6 CF® dye tyramides (CF®488A, CF®543, CF®568, CF®594, CF®640R, CF®680R) or biotin tyramide. CF® dyes are Biotium’s line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes.

Kit Components:

  • CF® dye or biotin-tyramide stock solution
  • HRP Goat anti-mouse IgG
  • BSA for blocking
  • Ready-to-Use Tyramide Amplification Buffer*
*Note: This product recently changed. For customers receiving kits with Tyramide Amplification buffer (Cat. No. 99822) and 30% H2O2 (Cat. No. 99823), please download the previous version of the product information sheet with protocol here: PI-Tyramide GARb Kits with with 99822 and 99823; The SDS for the previous version of the kit can be downloaded here: SDS Tyramide Amplification Kit 2018.

Also see our Tyramide Amplification Kits with HRP goat anti-rabbit and Tyramide Amplification Kits with HRP Streptavidin.

We also offer Ready-to-Use Tyramide Amplification Buffer, a large selection of CF® Dye Tyramides, and other tyramide compounds. See our full selection of tyramide products.

Tyramide Signal Amplification Kits

Tyramide LabelEx/EmSecondary conjugateCatalog no.
CF®488A490/515 nmGoat anti-mouse HRP33000
Goat anti-rabbit HRP33001
Streptavidin HRP33002
CF®543541/560 nmGoat anti-mouse HRP33003
Goat anti-rabbit HRP33004
Streptavidin HRP33005
CF®568562/583 nmGoat anti-mouse HRP33006
Goat anti-rabbit HRP33007
Streptavidin HRP33008
CF®594593/614 nmGoat anti-mouse HRP33009
Goat anti-rabbit HRP33010
Streptavidin HRP33011
CF®640R642/662 nmGoat anti-mouse HRP33012
Goat anti-rabbit HRP33013
Streptavidin HRP33014
CF®680R680/701 nmGoat anti-mouse HRP33015
Goat anti-rabbit HRP33016
Streptavidin HRP33017
Biotin-XXN/AGoat anti-mouse HRP33018
Goat anti-rabbit HRP33019
Streptavidin HRP33020
 

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