Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF® Dye or Biotin Tyramide
Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.
This kit contains reagents for performing tyramide signal amplification (TSA) using goat anti-mouse HRP secondary antibody and fluorescent CF® Dyes or biotin to increase immunofluorescence signal.
- Increase sensitivity by up to 100-fold
- Use lower primary antibody concentrations
- Boost signal in challenging samples, like human FFPE tissues
- Choose from 6 bright & photostable CF® dyes or biotin
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to conventional avidin-biotin detection. Multiple rounds of tyramide signal amplification can be performed for multiplex detection; to learn more, see our Tech Tip: Multicolor Fluorescence Imaging using Tyramide Amplification Kits. Learn more about tyramide signal amplification.
The HRP goat anti-mouse antibody provided in the kits has been adsorbed against bovine, horse, human, rabbit, and swine serum to minimize cross-reactivity; the antibody is not cross-adsorbed against rat. The kits include your choice of 6 CF® dye tyramides or biotin tyramide. CF® dyes are Biotium’s line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Learn more about CF® dyes.
Also see our Tyramide Amplification Kits with HRP Goat Anti-Rabbit and Tyramide Amplification Kits with HRP Streptavidin.
Each kit contains reagents for staining 50-150 slides; the actual number of samples that can be stained will depend on staining volumes used. Tyramide Amplification Buffer, CF® Dye Tyramides, and other tyramide compounds are also available separately. See our full selection of tyramide products.
Tyramide Signal Amplification Kits
|Tyramide Label||Ex/Em||Secondary conjugate||Catalog no.|
|CF®488A||490/515 nm||Goat anti-mouse HRP||33000|
|Goat anti-rabbit HRP||33001|
|CF®543||541/560 nm||Goat anti-mouse HRP||33003|
|Goat anti-rabbit HRP||33004|
|CF®568||562/583 nm||Goat anti-mouse HRP||33006|
|Goat anti-rabbit HRP||33007|
|CF®594||593/614 nm||Goat anti-mouse HRP||33009|
|Goat anti-rabbit HRP||33010|
|CF®640R||642/662 nm||Goat anti-mouse HRP||33012|
|Goat anti-rabbit HRP||33013|
|CF®680R||680/701 nm||Goat anti-mouse HRP||33015|
|Goat anti-rabbit HRP||33016|
|Biotin-XX||N/A||Goat anti-mouse HRP||33018|
|Goat anti-rabbit HRP||33019|