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CF® Dye Tyramide

Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

Product Attributes

Chemical reactivity (reacts with)

Tyrosine residues

Functional group

Tyramide

Applications

Immunofluorescence, Immunohistology (formalin), Immunohistology (frozen), In situ hybridization, Tissue staining

Storage Conditions

Store at -10 to -35 °C, Protect from light

Conjugation
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0.5 mg
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Product Description

CF® dye tyramide conjugates are used for tyramide signal amplification (TSA), a method for high-density labeling of a target protein or nucleic acid in situ. A fluorescent dye/biotin tyramide is incubated in the presence of an antibody- or streptavidin-horseradish peroxidase (HRP) conjugate which catalyzes binding of the tyramide (and associated label) to adjacent tyrosine residues for enhanced fluorescent signal. Multiple rounds of TSA can be performed for multiplex detection.

  • High-density labeling of a target protein or nucleic acid for enhanced immunofluorescence sensitivity
  • Especially suited for the detection of low abundance targets.
  • Detection sensitivity of over 100-fold compared to conventional procedures.
  • Enables multiplex multicolor detection, not limited by antibodies from the same host species.
  • Bright, photostable and water-soluble CF® dyes are excellent options for fluorescent labeling.

We also offer Ready-to-Use Tyramide Amplification Buffer, Tyramide Amplification Buffer Plus (an improved formulation for enhanced TSA sensitivity), and CF® Dye Tyramide Amplification Kits.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Advantages of Tyramide Signal Amplification (TSA)

TSA is a highly sensitive method for differential gene or protein analysis or detection of low-abundance targets, in fluorescent ICC, IHC, and FISH applications. An antibody- or streptavidin-HRP conjugate catalyzes the deposition of fluorescent dye/biotin tyramides on tyrosine residues on and adjacent to a target protein or nucleic acid sequence in situ. This results in high-density labeling of the target and significantly improves the detection sensitivity up to 100-fold compared to conventional methods. TSA is particularly advantageous for fluorescence detection in human tissue, where conventional ICC or FISH often fails to provide adequate signal over autofluorescence background. In applications where increased sensitivity is not required, TSA enables the use of significantly lower antibody or probe concentrations for the same level of detection sensitivity thereby reducing issues of non-specific binding or cross-reactivity. Furthermore, since binding of the (tyramide) label is covalent, a large number of targets can be detected in the same sample using multiple rounds of sequential TSA, in which the availability of antibodies from different host species is not a limitation. TSA also can be easily integrated with conventional immunostaining. Learn more about Tyramide Signal Amplification.

CF Dye Tyramides: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
CF350® TyramideCF®350347/448 nm0.5 mg92170Purchase 92170
CF®405S TyramideCF®405S404/431 nm0.5 mg92197Purchase 92197
CF®405M TyramideCF®405M408/452 nm0.5 mg96057Purchase 96057
CF®405L TyramideCF®405L395/545 nm0.5 mg92198Purchase 92198
CF®430 TyramideCF®430426/498 nm0.5 mg96053Purchase 96053
CF®488A TyramideCF®488A490/515 nm0.5 mg92171Purchase 92171
CF®514 TyramideCF®514516/548 nm0.5 mg92199Purchase 92199
CF®532 TyramideCF®532527/558 nm 0.5 mg96066Purchase 96066
CF®543 TyramideCF®543541/560 nm0.5 mg92172Purchase 92172
CF®550R TyramideCF®550R551/577 nm0.5 mg96077Purchase 96077
CF®555 TyramideCF®555555/565 nm0.5 mg96021Purchase 96021
CF®568 TyramideCF®568562/583 nm0.5 mg92173Purchase 92173
CF®594 TyramideCF®594593/614 nm0.5 mg92174Purchase 92174
CF®620R TyramideCF®620R617/639 nm0.5 mg92194Purchase 92194
CF®640R TyramideCF®640R642/662 nm0.5 mg92175Purchase 92175
CF®647 TyramideCF®647650/665 nm0.5 mg96022Purchase 96022
CF®660R TyramideCF®660R663/682 nm0.5 mg92195Purchase 92195
CF®680R TyramideCF®680R680/701 nm0.5 mg92196Purchase 92196
CF®750 TyramideCF®750755/777 nm0.5 mg96052Purchase 96052

References

1. Journal of Immunology Research (2020), 2020:2328675. DOI:10.1155/2020/2328675
2. Scientific Reports (2019), 9:1144. DOI:10.1038/s41598-018-38171-5
3. bioRxiv (2019) preprint. DOI:10.1101/651083
4. OncoImmunology (2019), 8(6):e1581528-10. DOI:10.1080/2162402X.2019.1581528
5. Microbiological Research (2018), 217: 69-80. DOI:10.1016/j.micres.2018.08.017
6. Mol Neurodegeneration (2017), 12:68. DOI:10.1186/s13024-017-0202-z
7. Biology Open (2017), 6: 891-896. DOI:10.1242/bio.025809
8. The American Journal of Pathology (2016), 186(10):2650-2664. DOI:10.1016/j.ajpath.2016.06.020

Download a list of CF® dye references.

 

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