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CF® Dye Tyramide

Fluorescent CF® Dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

Product Attributes

Chemical reactivity (reacts with)

Tyrosine residues

Functional group


Assay type/options

Tissue staining

Storage Conditions

Store at -10 to -35 °C, Protect from light

Detection method/readout

Fluorescence microscopy

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Catalog #
0.5 mg
Clear selection
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Product Description

CF® Dye tyramide conjugates are used for tyramide signal amplification (TSA), a method for high-density labeling of a target protein or nucleic acid in situ.

  • High-density labeling of a target protein or nucleic acid for enhanced immunofluorescence sensitivity
  • Especially suited for the detection of low abundance targets
  • Detection sensitivity of over 100-fold compared to conventional procedures
  • Enables multiplex multicolor detection, not limited by antibodies from the same host species
  • Wide selection of bright, photostable and water-soluble CF® Dyes, excellent options for fluorescent labeling
  • CF®754 Tyramide is a unique near-IR conjugate compatible with automated staining

We also offer Ready-to-Use Tyramide Amplification Buffer, Tyramide Amplification Buffer Plus (an improved formulation for enhanced TSA sensitivity), and CF® Dye Tyramide Amplification Kits.

Superior CF® Dyes

Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to other commercially available fluorescent dyes. Our CF® Dye Tyramide conjugates are available in 21 colors. Learn more about CF® Dyes.

CF®754 Tyramide: A Stable Near-IR Tyramide for Automated Staining

The stability of CF®754 Tyramide in the amplification buffer makes it suitable for staining in automated systems (ie. BOND RX) where the dye may be added to the buffer in advance for several hours to overnight without degradation. CF®754 Tyramide was developed as an alternative to CF®750 Tyramide, which is not stable in the oxidizing amplification buffer. CF®754 is stable in reaction buffer and can be used for automated staining. However, CF®750 Tyramide may still be used if added to the amplification buffer just before use. Near-IR tyramide background staining may be tissue dependent and not suitable for all targets. In general, we would recommend using near-IR staining for more abundant targets in your panel.

Tyramide Signal Amplification

TSA is a highly sensitive method for differential gene or protein analysis or detection of low-abundance targets, in fluorescent ICC, IHC, and FISH applications. An antibody- or streptavidin-HRP conjugate catalyzes the deposition of fluorescent dye/biotin tyramides on tyrosine residues on and adjacent to a target protein or nucleic acid sequence in situ. This results in high-density labeling of the target and significantly improves the detection sensitivity up to 100-fold compared to conventional methods. TSA is particularly advantageous for fluorescence detection in human tissue, where conventional ICC or FISH often fails to provide adequate signal over autofluorescence background. In applications where increased sensitivity is not required, TSA enables the use of significantly lower antibody or probe concentrations for the same level of detection sensitivity thereby reducing issues of non-specific binding or cross-reactivity. Furthermore, since binding of the tyramide label is covalent, a large number of targets can be detected in the same sample using multiple rounds of sequential TSA, in which the availability of antibodies from different host species is not a limitation. TSA also can be easily integrated with conventional immunostaining. Learn more about Tyramide Signal Amplification.

CF® Dye Tyramides

ProductEx/EmSizeCatalog No.
CF®350 Tyramide347/448 nm0.5 mg92170
CF®405S Tyramide404/431 nm0.5 mg92197
CF®405M Tyramide408/452 nm0.5 mg96057
CF®405L Tyramide395/545 nm0.5 mg92198
CF®430 Tyramide426/498 nm0.5 mg96053
CF®488A Tyramide490/515 nm0.5 mg92171
CF®514 Tyramide516/548 nm0.5 mg92199
CF®532 Tyramide527/558 nm0.5 mg96066
CF®543 Tyramide541/560 nm0.5 mg92172
CF®550R Tyramide551/577 nm0.5 mg96077
CF®555 Tyramide555/565 nm0.5 mg96021
CF®568 Tyramide562/583 nm0.5 mg92173
CF®583R Tyramide586/609 nm0.5 mg96085
CF®594 Tyramide593/614 nm0.5 mg92174
CF®620R Tyramide617/639 nm0.5 mg92194
CF®640R Tyramide642/662 nm0.5 mg92175
CF®647 Tyramide650/665 nm0.5 mg96022
CF®660R Tyramide663/682 nm0.5 mg92195
CF®680R Tyramide680/701 nm0.5 mg92196
CF®750 Tyramide*755/779 nm0.5 mg96052
CF®754 Tyramide748/793 nm0.5 mg96090

* CF®750 Tyramide is not stable in TSA buffer, and should be added to the buffer immediately before performing the staining reaction.


1. Journal of Immunology Research (2020), 2020:2328675. DOI:10.1155/2020/2328675
2. Scientific Reports (2019), 9:1144. DOI:10.1038/s41598-018-38171-5
3. bioRxiv (2019) preprint. DOI:10.1101/651083
4. OncoImmunology (2019), 8(6):e1581528-10. DOI:10.1080/2162402X.2019.1581528
5. Microbiological Research (2018), 217: 69-80. DOI:10.1016/j.micres.2018.08.017
6. Mol Neurodegeneration (2017), 12:68. DOI:10.1186/s13024-017-0202-z
7. Biology Open (2017), 6: 891-896. DOI:10.1242/bio.025809
8. The American Journal of Pathology (2016), 186(10):2650-2664. DOI:10.1016/j.ajpath.2016.06.020

Download a list of CF® Dye references.


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