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CF® Dye Tyramides

Fluorescent CF® dye tyramide conjugates for tyramide signal amplification (TSA). A horseradish peroxidase (HRP)-mediated method for high-density labeling of a target protein or nucleic acid in situ.

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Product Description

CF® dye tyramide conjugates can be used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. A tyramide conjugate is incubated in the presence of HRP (often bound to a secondary antibody), which catalyzes the binding of the tyramide (and associated label) to adjacent tyrosines for enhanced fluorescent signal. Multiple rounds of tyramide signal amplification can be performed for multiplex detection; to learn more, see our Tech Tip: Multicolor Fluorescence Imaging using Tyramide Amplification Kits.

We also offer Ready-to-Use Tyramide Amplification Buffer, and CF® Dye Tyramide Amplification Kits.

Learn more about tyramide signal amplification.

CF Dye Tyramides: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
CF350® TyramideCF®350347/448 nm0.5 mg92170Purchase 92170
CF®405L TyramideCF®405L395/545 nm0.5 mg92198Purchase 92198
CF®405S TyramideCF®405S404/431 nm0.5 mg92197Purchase 92197
CF®405M TyramideCF®405M408/452 nm0.5 mg96057Purchase 96057
CF®430 TyramideCF®430426/498 nm0.5 mg96053Purchase 96053
CF®488A TyramideCF®488A490/515 nm0.5 mg92171Purchase 92171
CF®514 TyramideCF®514516/548 nm0.5 mg92199Purchase 92199
CF®532 TyramideCF®532527/558 nm 0.5 mg96066Purchase 96066
CF®543 TyramideCF®543541/560 nm0.5 mg92172Purchase 92172
CF®555 TyramideCF®555555/565 nm0.5 mg96021Purchase 96021
CF®568 TyramideCF®568562/583 nm0.5 mg92173Purchase 92173
CF®594 TyramideCF®594593/614 nm0.5 mg92174Purchase 92174
CF®620R TyramideCF®620R617/639 nm0.5 mg92194Purchase 92194
CF®640R TyramideCF®640R642/662 nm0.5 mg92175Purchase 92175
CF®647 TyramideCF®647650/665 nm0.5 mg96022Purchase 96022
CF®660R TyramideCF®660R663/682 nm0.5 mg92195Purchase 92195
CF®680R TyramideCF®680R680/701 nm0.5 mg92196Purchase 92196
CF®750 TyramideCF®750755/777 nm0.5 mg96052Purchase 96052

References

1. Journal of Immunological Methods 125, 279-285 (1989).
2. Journal of Immunological Methods 137, 103-112 (1991).
3. Cytometry A. (2010), doi:10.1002/cyto.a.20970
4. Molecular Neurodegeneration (2017), doi: 10.1186/s13024-017-0202-z (CF568® Tyramide)

Download a list of CF® dye references.

 

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