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CF® Dye Tyramide

Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

Product Attributes

Chemical reactivity (reacts with)

Tyrosine residues

Functional group

Tyramide

Applications

Immunofluorescence, Immunohistology (formalin), Immunohistology (frozen), In situ hybridization, Tissue staining

Storage Conditions

Store at -10 to -35 °C, Protect from light

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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0.5 mg
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Product Description

CF® dye tyramide conjugates are used for tyramide signal amplification (TSA), a method for high-density labeling of a target protein or nucleic acid in situ.

  • High-density labeling of a target protein or nucleic acid for enhanced immunofluorescence sensitivity
  • Especially suited for the detection of low abundance targets
  • Detection sensitivity of over 100-fold compared to conventional procedures
  • Enables multiplex multicolor detection, not limited by antibodies from the same host species
  • Bright, photostable and water-soluble CF® dyes are excellent options for fluorescent labeling

We also offer Ready-to-Use Tyramide Amplification Buffer, Tyramide Amplification Buffer Plus (an improved formulation for enhanced TSA sensitivity), and CF® Dye Tyramide Amplification Kits.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Tyramide Signal Amplification

TSA is a highly sensitive method for differential gene or protein analysis or detection of low-abundance targets, in fluorescent ICC, IHC, and FISH applications. An antibody- or streptavidin-HRP conjugate catalyzes the deposition of fluorescent dye/biotin tyramides on tyrosine residues on and adjacent to a target protein or nucleic acid sequence in situ. This results in high-density labeling of the target and significantly improves the detection sensitivity up to 100-fold compared to conventional methods. TSA is particularly advantageous for fluorescence detection in human tissue, where conventional ICC or FISH often fails to provide adequate signal over autofluorescence background. In applications where increased sensitivity is not required, TSA enables the use of significantly lower antibody or probe concentrations for the same level of detection sensitivity thereby reducing issues of non-specific binding or cross-reactivity. Furthermore, since binding of the tyramide label is covalent, a large number of targets can be detected in the same sample using multiple rounds of sequential TSA, in which the availability of antibodies from different host species is not a limitation. TSA also can be easily integrated with conventional immunostaining. Learn more about Tyramide Signal Amplification.

CF® Dye Tyramides

ProductEx/EmSizeSKUCatalog No.
CF350® Tyramide347/448 nm0.5 mg9217092170
CF®405S Tyramide404/431 nm0.5 mg9219792197
CF®405M Tyramide408/452 nm0.5 mg9605796057
CF®405L Tyramide395/545 nm0.5 mg9219892198
CF®430 Tyramide426/498 nm0.5 mg9605396053
CF®488A Tyramide490/515 nm0.5 mg9217192171
CF®514 Tyramide516/548 nm0.5 mg9219992199
CF®532 Tyramide527/558 nm0.5 mg9606696066
CF®543 Tyramide541/560 nm0.5 mg9217292172
CF®550R Tyramide551/577 nm0.5 mg9607796077
CF®555 Tyramide555/565 nm0.5 mg9602196021
CF®568 Tyramide562/583 nm0.5 mg9217392173
CF®583R Tyramide586/609 nm0.5 mg9608596085
CF®594 Tyramide593/614 nm0.5 mg9217492174
CF®620R Tyramide617/639 nm0.5 mg9219492194
CF®640R Tyramide642/662 nm0.5 mg9217592175
CF®647 Tyramide650/665 nm0.5 mg9602296022
CF®660R Tyramide663/682 nm0.5 mg9219592195
CF®680R Tyramide680/701 nm0.5 mg9219692196
Note: CF®750 tyramide (catalog #96052) has been discontinued. Current users may contact Technical Support to inquire about availability of remaining stock.

References

1. Journal of Immunology Research (2020), 2020:2328675. DOI:10.1155/2020/2328675
2. Scientific Reports (2019), 9:1144. DOI:10.1038/s41598-018-38171-5
3. bioRxiv (2019) preprint. DOI:10.1101/651083
4. OncoImmunology (2019), 8(6):e1581528-10. DOI:10.1080/2162402X.2019.1581528
5. Microbiological Research (2018), 217: 69-80. DOI:10.1016/j.micres.2018.08.017
6. Mol Neurodegeneration (2017), 12:68. DOI:10.1186/s13024-017-0202-z
7. Biology Open (2017), 6: 891-896. DOI:10.1242/bio.025809
8. The American Journal of Pathology (2016), 186(10):2650-2664. DOI:10.1016/j.ajpath.2016.06.020

Download a list of CF® dye references.

 

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