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Buffers & Accessories

For added convenience, Biotium offers a wide range of accessory products.

TrueBlack® lipofuscin autofluroescence quencher is the superior quencher for tissue section immunofluorescence.

EverBrite™ Mounting Medium is an excellent antifade for fluorescence microscopy and comes in wetset and hardset versions.

CoverGrip™ coverslip sealant and SuperHT Pap pens for microscopy slides.

Buffers (ie, 5X TBE, 10X PBS, Luciferase lysis buffers, Annexin V binding buffer).

Blocking reagents (ie, fish gelatin, dry milk, BSA).

Others: fixation and permeabilization reagents, reducing reagents, apoptosis inducers, and other chemicals.

Showing 1–20 of 40 results

    10X Fish Gelatin Blocking Agent

    From: $56 Sizes: 100 mLCatalog #: 22010

    10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

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    100 mL
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    10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

     

    10X Phosphate-Buffered Saline (PBS)

    From: $135 Sizes: 4 LCatalog #: 22020

    Molecular biology grade 10X phosphate-buffered saline (PBS). RNase-, DNase-, protease-free.

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    4 L
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    Molecular biology grade 10X phosphate-buffered saline (PBS). RNase-, DNase-, protease-free.

     

    5X Annexin V Binding Buffer

    From: $27 Sizes: 15 mLCatalog #: 99902

    Concentrated Annexin V Binding Buffer to be used for Annexin V staining.

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    15 mL
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    Concentrated Annexin V Binding Buffer to be used for Annexin V staining.

     

    5X Firefly Luciferase Lysis Buffer

    From: $18 Sizes: 15 mLCatalog #: 99923

    5X Firefly Lysis Buffer is a concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kit  and Firefly Luciferase Assay Kit (Lyophilized).

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    15 mL
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    5X Firefly Lysis Buffer is a concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kit  and Firefly Luciferase Assay Kit (Lyophilized).

     

    5X Passive Lysis Buffer

    From: $24 Sizes: 30 mLCatalog #: 99912

    5X Passive Lysis Buffer is concentrated lysis buffer designed for use with Biotium’s Renilla Luciferase Assay Kit (30004) or Firefly and Renilla Dual Luciferase Assay Kit (30005).

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    30 mL
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    5X Passive Lysis Buffer is concentrated lysis buffer designed for use with Biotium’s Renilla Luciferase Assay Kit (30004) or Firefly and Renilla Dual Luciferase Assay Kit (30005).

     

    Ac-DEVD-CHO Caspase-3 Inhibitor

    From: $68 Sizes: 1 mg, 5 mgCatalog #: 10404, - 10404-1View allHide

    Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.

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    Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.

     

    Bovine Serum Albumin 30% solution

    From: $89 Sizes: 100 mLCatalog #: 22014

    Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins such as antibodies and enzymes, and as a blocking agent to reduce non-specific protein binding in immunoassays such as ELISA, immunofluorescence staining, and Western blotting. This is a 30% solution of BSA in water, prepared using IgG- and protease-free BSA.

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    100 mL
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    Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins such as antibodies and enzymes, and as a blocking agent to reduce non-specific protein binding in immunoassays such as ELISA, immunofluorescence staining, and Western blotting. This is a 30% solution of BSA in water, prepared using IgG- and protease-free BSA.

     

    Bovine Serum Albumin Fraction V

    From: $137 Sizes: 50 gCatalog #: 22013

    Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody and use as a blocking agent to reduce non-specific binding. This BSA is protease- and IgG-free.

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    50 g
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    Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody and use as a blocking agent to reduce non-specific binding. This BSA is protease- and IgG-free.

     

    Calcium Calibration Buffer Kit

    From: $137 Sizes: 1 kitCatalog #: 59100

    This kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca2 indicators (1,2). This product contains 50 mL each of components A and B.

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    This kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca2 indicators (1,2). This product contains 50 mL each of components A and B.

     

    CoverGrip Coverslip Sealant

    From: $33 Sizes: 15 mL, 100 mLCatalog #: 23005, - 23005-1View allHide

    CoverGrip™ Coverslip Sealant is the first product specifically designed for sealing the edges of wet-mounted coverslips for fluorescence microscopy. Unlike nail polish, CoverGrip™ contains no ingredients that can leach into aqueous mounting medium and affect specimen fluorescence.

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    15 mL
    100 mL
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    CoverGrip™ Coverslip Sealant is the first product specifically designed for sealing the edges of wet-mounted coverslips for fluorescence microscopy. Unlike nail polish, CoverGrip™ contains no ingredients that can leach into aqueous mounting medium and affect specimen fluorescence.

     

    DMSO, anhydrous

    From: $21 Sizes: 10 mLCatalog #: 90082

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

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    10 mL
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    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

     

    dNTP Mix, 10 mM each

    From: $368 Sizes: 5 x 1 mLCatalog #: 40054

    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.

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    5 x 1 mL
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    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.

     

    dNTP Mix, 25 mM each

    From: $189 Sizes: 1 mLCatalog #: 40053

    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.

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    1 mL
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    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.

     

    dNTP Set, 100 mM each

    From: $189 Sizes: 1 set - 250 uL eachCatalog #: 40052

    Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

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    1 set - 250 uL each
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    Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

     

    Dry Milk Powder

    From: $48 Sizes: 4 x 25 gCatalog #: 22012

    Nonfat dry milk is commonly added to blocking buffers minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Milk can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.

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    4 x 25 g
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    Nonfat dry milk is commonly added to blocking buffers minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Milk can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.

     

    DTT

    From: $32 Sizes: 1 gCatalog #: 91050

    DTT

    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

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    1 g
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    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

     

    EDC (EDAC)

    From: $69 Sizes: 100 mgCatalog #: 59002

    EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.

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    100 mg
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    EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.

     

    EverBrite Hardset Mounting Medium

    From: $131 Sizes: 10 mLCatalog #: 23003, - 23004View allHide

    Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. EverBrite™ Hardset mounting medium hardens at room temperature completely in 24 hours.

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    10 mL
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    Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. EverBrite™ Hardset mounting medium hardens at room temperature completely in 24 hours.

     

    EverBrite Mounting Medium

    From: $116 Sizes: 10 mLCatalog #: 23001, - 23002View allHide

    Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. The mounting medium permits immediate imaging of slides and tissues after sealing edges of coverslips with nail polish or Biotium’s CoverGrip™ Coverslip Sealant.

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    Qty
    10 mL
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    Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. The mounting medium permits immediate imaging of slides and tissues after sealing edges of coverslips with nail polish or Biotium’s CoverGrip™ Coverslip Sealant.

     

    Fish Gelatin Powder

    From: $50 Sizes: 2 x 50 gCatalog #: 22011

    Fish gelatin is commonly used in blocking solutions to minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Because this gelatin is derived from the skin of cold water fish it does not cross-react with mammalian antibodies.

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    Qty
    2 x 50 g
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    Fish gelatin is commonly used in blocking solutions to minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Because this gelatin is derived from the skin of cold water fish it does not cross-react with mammalian antibodies.