Buffers & Accessories
Showing 1–20 of 37 results
10X Fish Gelatin Blocking Agent
10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.
10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.
5X Annexin V Binding Buffer
Concentrated Annexin V Binding Buffer to be used for Annexin V staining.
Concentrated Annexin V Binding Buffer to be used for Annexin V staining.
5X Firefly Luciferase Lysis Buffer
5X Firefly Lysis Buffer is a concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kit and Firefly Luciferase Assay Kit (Lyophilized).
5X Firefly Lysis Buffer is a concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kit and Firefly Luciferase Assay Kit (Lyophilized).
5X Passive Lysis Buffer
5X Passive Lysis Buffer is concentrated lysis buffer designed for use with Biotium’s Renilla Luciferase Assay Kit (30004) or Firefly and Renilla Dual Luciferase Assay Kit (30005).
5X Passive Lysis Buffer is concentrated lysis buffer designed for use with Biotium’s Renilla Luciferase Assay Kit (30004) or Firefly and Renilla Dual Luciferase Assay Kit (30005).
Ac-DEVD-CHO Caspase-3 Inhibitor
Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.
Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.
Bovine Serum Albumin 30% solution
Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins such as antibodies and enzymes, and as a blocking agent to reduce non-specific protein binding in immunoassays such as ELISA, immunofluorescence staining, and Western blotting. This is a 30% solution of BSA in water, prepared using IgG- and protease-free BSA.
Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins such as antibodies and enzymes, and as a blocking agent to reduce non-specific protein binding in immunoassays such as ELISA, immunofluorescence staining, and Western blotting. This is a 30% solution of BSA in water, prepared using IgG- and protease-free BSA.
Bovine Serum Albumin Fraction V
Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody and use as a blocking agent to reduce non-specific binding. This BSA is protease- and IgG-free.
Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody and use as a blocking agent to reduce non-specific binding. This BSA is protease- and IgG-free.
Calcium Calibration Buffer Kit
This kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca2 indicators (1,2). This product contains 50 mL each of components A and B.
This kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca2 indicators (1,2). This product contains 50 mL each of components A and B.
CoverGrip Coverslip Sealant
CoverGrip™ Coverslip Sealant is the first product specifically designed for sealing the edges of wet-mounted coverslips for fluorescence microscopy. Unlike nail polish, CoverGrip™ contains no ingredients that can leach into aqueous mounting medium and affect specimen fluorescence.
CoverGrip™ Coverslip Sealant is the first product specifically designed for sealing the edges of wet-mounted coverslips for fluorescence microscopy. Unlike nail polish, CoverGrip™ contains no ingredients that can leach into aqueous mounting medium and affect specimen fluorescence.
DMSO, anhydrous
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
dNTP Mix, 10 mM each
Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.
Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.
dNTP Mix, 25 mM each
Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.
Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.
dNTP Set, 100 mM each
Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.
Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.
Dry Milk Powder
Nonfat dry milk is commonly added to blocking buffers minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Milk can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.
Nonfat dry milk is commonly added to blocking buffers minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Milk can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.
DTT
DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.
DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.
EDC (EDAC)
EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.
EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.
EverBrite Hardset Mounting Medium
Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF™ dyes and other fluorochromes. EverBrite™ Hardset mounting medium hardens at room temperature completely in 24 hours.
Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF™ dyes and other fluorochromes. EverBrite™ Hardset mounting medium hardens at room temperature completely in 24 hours.
EverBrite Mounting Medium
Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF™ dyes and other fluorochromes. The mounting medium permits immediate imaging of slides and tissues after sealing edges of coverslips with nail polish or Biotium’s CoverGrip™ Coverslip Sealant.
Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF™ dyes and other fluorochromes. The mounting medium permits immediate imaging of slides and tissues after sealing edges of coverslips with nail polish or Biotium’s CoverGrip™ Coverslip Sealant.
Fish Gelatin Powder
Fish gelatin is commonly used in blocking solutions to minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Because this gelatin is derived from the skin of cold water fish it does not cross-react with mammalian antibodies.
Fish gelatin is commonly used in blocking solutions to minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Because this gelatin is derived from the skin of cold water fish it does not cross-react with mammalian antibodies.
Fixation Buffer
Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry. Also available in the Flow Cytometry Fixation/Permeabilization Kit (cat. 23006).
Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry. Also available in the Flow Cytometry Fixation/Permeabilization Kit (cat. 23006).