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Buffers & Accessories

Showing 1–20 of 39 results

    10X Fish Gelatin Blocking Agent

    Sizes: 100 mLCatalog #: 22010

    10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

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    100 mL
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    10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

     

    5X Annexin V Binding Buffer

    From: $27 Sizes: 15 mLCatalog #: 99902

    Concentrated Annexin V Binding Buffer to be used for Annexin V staining.

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    15 mL
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    Concentrated Annexin V Binding Buffer to be used for Annexin V staining.

     

    5X Firefly Luciferase Lysis Buffer

    From: $18 Sizes: 15 mLCatalog #: 99923

    5X Firefly Lysis Buffer is a concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kit  and Firefly Luciferase Assay Kit (Lyophilized).

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    15 mL
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    5X Firefly Lysis Buffer is a concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kit  and Firefly Luciferase Assay Kit (Lyophilized).

     

    5X Passive Lysis Buffer

    From: $24 Sizes: 30 mLCatalog #: 99912

    5X Passive Lysis Buffer is concentrated lysis buffer designed for use with Biotium’s Renilla Luciferase Assay Kit (30004) or Firefly and Renilla Dual Luciferase Assay Kit (30005).

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    30 mL
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    5X Passive Lysis Buffer is concentrated lysis buffer designed for use with Biotium’s Renilla Luciferase Assay Kit (30004) or Firefly and Renilla Dual Luciferase Assay Kit (30005).

     

    Ac-DEVD-CHO Caspase-3 Inhibitor

    From: $68 Sizes: 1 mg, 5 mgCatalog #: 10404, - 10404-1View allHide

    Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.

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    Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.

     

    AccuEasy Flow Cytometry Kit

    From: $209 Sizes: 1 kitCatalog #: 30069

    Conventional methods for analyzing cell surface markers on adherent cells by flow cytometry require detachment of cells from their culture surface before antibody staining. Unfortunately, cell detachment introduces a significant stress to cells, which can lead to changes in expression of cell surface markers.

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    Conventional methods for analyzing cell surface markers on adherent cells by flow cytometry require detachment of cells from their culture surface before antibody staining. Unfortunately, cell detachment introduces a significant stress to cells, which can lead to changes in expression of cell surface markers.

     

    Bovine Serum Albumin 30% solution

    Sizes: 100 mLCatalog #: 22014

    Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins such as antibodies and enzymes, and as a blocking agent to reduce non-specific protein binding in immunoassays such as ELISA, immunofluorescence staining, and Western blotting. This is a 30% solution of BSA in water, prepared using IgG- and protease-free BSA.

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    100 mL
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    Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins such as antibodies and enzymes, and as a blocking agent to reduce non-specific protein binding in immunoassays such as ELISA, immunofluorescence staining, and Western blotting. This is a 30% solution of BSA in water, prepared using IgG- and protease-free BSA.

     

    Bovine Serum Albumin Fraction V

    From: $130 Sizes: 50 gCatalog #: 22013

    Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody and use as a blocking agent to reduce non-specific binding. It is also suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization.

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    50 g
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    Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody and use as a blocking agent to reduce non-specific binding. It is also suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization.

     

    Calcium Calibration Buffer Kit

    Sizes: 1 kitCatalog #: 59100

    This kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca2 indicators (1,2). This product contains 50 mL each of components A and B.

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    This kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca2 indicators (1,2). This product contains 50 mL each of components A and B.

     

    CoverGrip Coverslip Sealant

    From: $33 Sizes: 15 mL, 100 mLCatalog #: 23005, - 23005-1View allHide

    CoverGrip™ Coverslip Sealant is the first product specifically designed for sealing the edges of wet-mounted coverslips for fluorescence microscopy. Unlike nail polish, CoverGrip™ contains no ingredients that can leach into aqueous mounting medium and affect specimen fluorescence.

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    15 mL
    100 mL
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    CoverGrip™ Coverslip Sealant is the first product specifically designed for sealing the edges of wet-mounted coverslips for fluorescence microscopy. Unlike nail polish, CoverGrip™ contains no ingredients that can leach into aqueous mounting medium and affect specimen fluorescence.

     

    DMSO, anhydrous

    Sizes: 10 mLCatalog #: 90082

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

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    10 mL
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    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

     

    DNA Gel Extraction Kit

    From: $95 Sizes: 50 assays, 250 assaysCatalog #: 31030-250, - 31030-50View allHide

    Biotium’s DNA Gel Extraction Kit is a silica-gel spin column based DNA extraction kit designed to purify 40 bp-40 kb DNA fragments from agarose gels in TAE or TBE buffer. Each column has a maximum binding capacity of 10 ug DNA.

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    50 assays
    250 assays
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    Biotium’s DNA Gel Extraction Kit is a silica-gel spin column based DNA extraction kit designed to purify 40 bp-40 kb DNA fragments from agarose gels in TAE or TBE buffer. Each column has a maximum binding capacity of 10 ug DNA.

     

    dNTP Mix, 10 mM each

    From: $368 Sizes: 5 x 1 mLCatalog #: 40054

    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.

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    5 x 1 mL
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    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.

     

    dNTP Mix, 25 mM each

    From: $189 Sizes: 1 mLCatalog #: 40053

    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.

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    1 mL
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    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.

     

    dNTP Set, 100 mM each

    From: $189 Sizes: 1 set - 250 uL eachCatalog #: 40052

    Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

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    1 set - 250 uL each
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    Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

     

    Dry Milk Powder

    Sizes: 4 x 25 gCatalog #: 22012

    Nonfat dry milk is commonly added to blocking buffers minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Milk can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.

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    4 x 25 g
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    Nonfat dry milk is commonly added to blocking buffers minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Milk can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.

     

    DTT

    Sizes: 1 gCatalog #: 91050

    DTT

    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

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    1 g
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    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

     

    EDC (EDAC)

    Sizes: 100 mgCatalog #: 59002

    EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.

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    100 mg
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    EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.

     

    EverBrite Hardset Mounting Medium

    From: $131 Sizes: 10 mLCatalog #: 23003, - 23004View allHide

    Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF™ dyes and other fluorochromes. EverBrite™ Hardset mounting medium hardens at room temperature completely in 24 hours.

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    10 mL
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    Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF™ dyes and other fluorochromes. EverBrite™ Hardset mounting medium hardens at room temperature completely in 24 hours.

     

    EverBrite Mounting Medium

    From: $116 Sizes: 10 mLCatalog #: 23001, - 23002View allHide

    Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF™ dyes and other fluorochromes. The mounting medium permits immediate imaging of slides and tissues after sealing edges of coverslips with nail polish or Biotium’s CoverGrip™ Coverslip Sealant.

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    10 mL
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    Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF™ dyes and other fluorochromes. The mounting medium permits immediate imaging of slides and tissues after sealing edges of coverslips with nail polish or Biotium’s CoverGrip™ Coverslip Sealant.