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EverBrite™ Hardset Mounting Medium

A revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. EverBrite™ Hardset mounting medium hardens at room temperature completely in 24 hours.

Product Attributes


Hardset, Without DAPI, With DAPI

For live or fixed cells

For fixed cells/tissue sections

Storage Conditions

Store at 2 to 8 °C, Protect from light

Cellular localization


Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Catalog #
10 mL
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Product Description

Biotium’s EverBrite™ line of mounting media are a revolutionary antifade mounting media optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. EverBrite™ Hardset mounting medium hardens at room temperature completely in 24 hours with excellent protection against photobleaching of fluorescent dyes. Unlike VectaShield®, EverBrite™ is compatible with cyanine-based fluorophores such as Cy®3 and Cy®5, Alexa Fluor® 647, and DyLight® 649.

  • Antifade mounting medium optimally formulated to prevent photobleaching of fluorophores
  • Hardens completely at room temperature in 24 hours
  • Refractive index is well-matched to that of coverslip glass and immersion oil
  • Cures to form a hard, permanent seal with the coverslip
  • No need to seal coverslip edges nail polish or other sealants
  • Available with or without DAPI

EverBrite™ Hardset mounting medium cures to form a hard, permanent seal with the coverslip at room temperature in 24 hours. This facilitates handling of the slide and eliminates the need to seal coverslip edges with nail polish or other sealants. It is optimally formulated for use with Biotum’s CF® dye conjugates but also provides a high level of protection from rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes. Mounted slides can be stored at 4°C for a year or longer. EverBrite™ Hardset Mounting Medium has a refractive index of 1.38 initially, which increases to 1.42 after 24 hours of curing, and 1.46 after four days of curing, after which it remains constant. EverBrite™ Hardset Mounting Medium is available with or without DAPI for nuclear counterstaining. Mounting media with DAPI gives a blue fluorescent nuclear counterstain without an additional staining step.

We also offer EverBrite™ Mounting Media in wet-set formulation, with or without DAPI. Also see our other accessory products for cell biology research.

EverBrite™ Mounting Media

ProductCounterstainCat. No.
EverBrite™ Mounting MediumNone23001
EverBrite™ Mounting Medium with DAPIDAPI23002
Drop-n-Stain EverBrite™ Mounting MediumNone23008
Drop-n-Stain EverBrite™ Mounting Medium with DAPIDAPI23009
EverBrite™ Hardset Mounting MediumNone23003
EverBrite™ Hardset Mounting Medium with DAPIDAPI23004


EverBrite is a trademark of Biotium, Inc.; CF is a registered trademark of Biotium, Inc.; Vectashield is a registered trademark of VectorLabs; Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy and CyDye are registered trademarks of GE Healthcare.



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