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5X Annexin V Binding Buffer

Concentrated buffer used for Annexin V staining of cells to monitor apoptosis.

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Store at 2 to 8 °C

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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

5X Annexin V Binding Buffer is used to promote optimal Annexin V staining of apoptotic cells.

  • Buffer optimized for Annexin V staining of apoptotic cells
  • Provided as a 5X concentrate
  • Dilute to 1X in water for use during and after Annexin V staining

Detect Apoptosis with Annexin V

Annexin V is a 35-36 kDa protein that has a high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasmic membrane, making itself available for Annexin V binding.

Biotium offers Annexin V conjugated to a wide range of CF® Dyes and other fluorescent dyes, including our unique range of near-infrared CF® Dyes. We also provide azide-free Annexin V conjugates for live cell staining.

Annexin V conjugates

ProductEx/EmConcentrationSizeCatalog No.
Biotin Annexin VN/A50 ug/mL0.5 mLPurchase 29013
CF®350 Annexin V 347/448 nm50 ug/mL0.5 mLPurchase 29012
CF®405M Annexin V 408/452 nm50 ug/mL0.5 mLPurchase 29009
CF®450 Annexin V 450/538 nm50 ug/mL0.5 mLPurchase 29083
CF®488A Annexin V 490/515 nm50 ug/mL0.5 mLPurchase 29005
CF®555 Annexin V 555/565 nm50 ug/mL0.5 mLPurchase 29004
CF®568 Annexin V 562/583 nm50 ug/mL0.5 mLPurchase 29010
CF®583R Annexin V 586/609 nm50 ug/mL0.5 mLPurchase 29085
CF®594 Annexin V 593/614 nm50 ug/mL0.5 mLPurchase 29011
CF®633 Annexin V 630/650 nm50 ug/mL0.5 mLPurchase 29008
CF®640R Annexin V 642/662 nm50 ug/mL0.5 mLPurchase 29014
CF®647 Annexin V 650/665 nm50 ug/mL0.5 mLPurchase 29003
CF®660R Annexin V 663/682 nm50 ug/mL0.5 mLPurchase 29069
R-PE Annexin V 496, 546,
565/578 nm
5 uL/test100 uLPurchase 29045-100uL
500 uLPurchase 29045-500uL
APC Annexin V 650/660 nm5 uL/test100 uLPurchase 29057-100uL
500 uLPurchase 29057-500uL
FITC Annexin V 496/516 nm50 ug/mL0.5 mLPurchase 29001
5X Annexin V Binding BufferN/A5X15 mLPurchase 99902

References

See a list of CF® Dye Annexin V references in the Supporting Documents tab.

 

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