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EverBrite™ Mounting Medium

A revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. Available with or without DAPI.

Product Attributes

Format

With DAPI, Without DAPI

For live or fixed cells

For fixed cells/tissue sections

Applications

Mounting medium

Format
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes for your immunofluorescence (IF) imaging applications.

  • Antifade mounting medium optimally formulated to prevent photobleaching of fluorophores
  • Refractive index is well-matched to that of coverslip glass and immersion oil
  • Available with or without DAPI

EverBrite™ provides a high level of protection from photobleaching to prolong fluorescence over a wide range of wavelengths. EverBrite™ Mounting Medium has a refractive index of 1.46, which is well-matched to the refractive indices of coverslip glass and immersion oil (1.5). The mounting medium remains liquid and permits immediate imaging of slides and tissues after sealing the cover slip edges with nail polish or Biotium’s CoverGrip™ Coverslip Sealant. After sealing, the slides can be stored at 4°C for a year or longer. EverBrite is available with or without DAPI for nuclear counterstaining. Unlike VectaShield®, EverBrite™ is compatible with cyanine-based fluorophores like Cy® dyes.

We also offer EverBrite™ Hardset Mounting Media with or without DAPI. Also see our other accessory products for cell biology research.

Photostability of FITC staining in HeLa cells with various mounting media. EverBrite™ Mounting Medium prolongs FITC fluorescence significantly when compared with SlowFade® Gold Antifade Mountant (Thermo Fisher Scientific).
Photostability of Cy®5 staining in HeLa cells with various mounting media. A major advantage of EverBrite™ mounting medium is that, unlike Vectashield®, it does not decrease the fluorescence of cyanine-based fluorophores. Fluorescence values for Cy®5 in Vectashield® media are normalized to PBS time t0 to illustrate the drop in fluorescence of cyanine dyes caused by Vectashield®.

EverBrite™ Mounting Media

ProductCounterstainCat. No.
EverBrite™ Mounting MediumNone23001
EverBrite™ Mounting Medium with DAPIDAPI23002
Drop-n-Stain EverBrite™ Mounting MediumNone23008
Drop-n-Stain EverBrite™ Mounting Medium with DAPIDAPI23009
EverBrite™ Hardset Mounting MediumNone23003
EverBrite™ Hardset Mounting Medium with DAPIDAPI23004
EverBrite is a trademark of Biotium, Inc.; CF is a registered trademark of Biotium, Inc.; VectaShield is a registered trademark of Vector Laboratories. Cy Dye is a registered trademark of GE Healthcare.

References

Cat#23001

  1. Mol Cell Probes, 101530, (2020), DOI: 10.1016/j.mcp.2020.101530
  2. Kidney Int, 95, 455, (2019), DOI: 10.1016/j.kint.2018.08.038
  3. Cell Mol Med, 23, 2890, (2019), DOI: 10.1111/jcmm.14172
  4. Molecular Neurobiology (2018), DOI: 10.1007/s12035-018-0971-6
  5. Neurobiology of Aging (2017), DOI: 10.1016/j.neurobiolaging.2017.03.005
  6. The Journal of Cell Biology (2017), DOI: 10.1083/jcb.201704171
  7. J Physiol (2016), DOI: 10.1113/JP271441
  8. Nature Communications (2016), DOI: 10.1038/ncomms10833
  9. Molecular Reproduction & Development (2015), DOI: 10.1002/mrd.22477
  10. British J Pharmacol. (2015), DOI: 10.1111/bph.13216
  11. GLIA (2015), DOI: 10.1002/glia.22873
  12. Mol. Immunol. (2015), DOI: 10.1016/j.molimm.2015.05.015
  13. Journal of Neurotrauma (2015), DOI: 10.1089/neu.2014.3517
  14. Histochem Cell Biol (2015), DOI: 10.1007/s00418-015-1380-9
  15. Journal of Neurotrauma (2014), DOI: 10.1089/neu.2014.3517
  16. Cell (2013), DOI: 10.1016/j.cell.2013.10.018

Cat#23002

  1. J Biomed Sci 27, 45 (2020), DOI: 10.1186/s12929-020-00633-2
  2. Arch Insect Biochem Physiol, e21678, (2020), DOI: 10.1002/arch.21678
  3. Arch Insect Biochem Physiol, e21677, (2020), DOI: 10.1002/arch.21677
  4. Nucleic Acids Research (2019), DOI: 10.1093/nar/gkz195
  5. PLoS Genet. (2017), DOI: 10.1371/journal.pgen.1006603
  6. Neurogastroenterology & Motility (2017), DOI: 10.1111/nmo.13157
  7. Insect Biochemistry and Molecular Biology (2017), DOI: 10.1016/j.ibmb.2017.09.011
 

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