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EverBrite™ Mounting Medium

A revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. Available with or without DAPI.

Product Attributes

Format

With DAPI, Without DAPI

For live or fixed cells

For fixed cells/tissue sections

Applications

Mounting medium

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10 mL
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Product Description

Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes for your immunofluorescence (IF) imaging applications.

  • Antifade mounting medium optimally formulated to prevent photobleaching of fluorophores
  • Refractive index is well-matched to that of coverslip glass and immersion oil
  • Available with or without DAPI

EverBrite™ provides a high level of protection from photobleaching to prolong fluorescence over a wide range of wavelengths. EverBrite™ Mounting Medium has a refractive index of 1.46, which is well-matched to the refractive indices of coverslip glass and immersion oil (1.5). The mounting medium remains liquid and permits immediate imaging of slides and tissues after sealing the cover slip edges with nail polish or Biotium’s CoverGrip™ Coverslip Sealant. After sealing, the slides can be stored at 4°C for a year or longer. EverBrite is available with or without DAPI for nuclear counterstaining. Unlike VectaShield®, EverBrite™ is compatible with cyanine-based fluorophores like Cy® dyes.

We also offer EverBrite™ Hardset Mounting Media with or without DAPI. Also see our other accessory products for cell biology research.

Photostability of FITC staining in HeLa cells with various mounting media. EverBrite™ Mounting Medium prolongs FITC fluorescence significantly when compared with SlowFade® Gold Antifade Mountant (Thermo Fisher Scientific).
Photostability of Cy®5 staining in HeLa cells with various mounting media. A major advantage of EverBrite™ mounting medium is that, unlike Vectashield®, it does not decrease the fluorescence of cyanine-based fluorophores. Fluorescence values for Cy®5 in Vectashield® media are normalized to PBS time t0 to illustrate the drop in fluorescence of cyanine dyes caused by Vectashield®.

EverBrite Mounting Media

ProductCounterstainCat. No.
EverBrite™ Mounting MediumNone23001
EverBrite™ Mounting Medium with DAPIDAPI23002
Drop-n-Stain EverBrite™ Mounting MediumNone23008
Drop-n-Stain EverBrite™ Mounting Medium with DAPIDAPI23009
EverBrite™ Hardset Mounting MediumNone23003
EverBrite™ Hardset Mounting Medium with DAPIDAPI23004
EverBrite is a trademark of Biotium, Inc.; CF is a registered trademark of Biotium, Inc.; VectaShield is a registered trademark of Vector Laboratories. Cy Dye is a registered trademark of GE Healthcare.

References

Cat#23001
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7. Journal of Neurotrauma (2015), DOI: 10.1089/neu.2014.3517
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9. J Physiol (2016), DOI: 10.1113/JP271441
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Cat#23002
1. Nucleic Acids Research (2019), DOI: 10.1093/nar/gkz195
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Cat#23003
1. Molecular Microbiology (2017), DOI: 10.1111/mmi.13761
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3. Molecular Neurobiology (2018), DOI: 10.1007/s12035-018-1030-z
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Cat#23004
1. ACS Chem. Neurosci. (2015), DOI: 10.1021/acschemneuro.5b00266
2. Nucleic Acid (2017), DOI: 10.1016/j.omtn.2017.06.019
3. npj Microgravity (2017), DOI: 10.1038/s41526-017-0032-x
4. Diabetes (2017), DOI: 10.2337/db17-0391
5. Journal of Developmental Neuroscience (2018), DOI: 10.1016/j.ijdevneu.2018.03.003
6. Nucleic Acid Therapeutics (2018), DOI: 10.1089/nat.2017.0705
7. Cancer Letters (2018), DOI: 10.1016/j.canlet.2018.06.023
8. Journal of Dental Sciences (2019), DOI: 10.1016/j.jds.2019.01.013

 

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