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Drop-n-Stain EverBrite™ Mounting Medium

Wet-set antifade mounting medium for preserving fluorescence of our CF® dyes and other fluorochromes, in a dropper bottle for easy dispensing.

Product Attributes

Format

Wet-set, Without DAPI, With DAPI

For live or fixed cells

For fixed cells/tissue sections

Probe cellular localization

Nucleus

Storage Conditions

Store at -10 to -35 °C

Format
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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10 mL
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Product Description

Drop-n-Stain EverBrite™ Mounting Medium is a wet-set antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes.  The dropper bottle makes for quick and easy dispensing.

  • Convenient dropper bottle for pipette-free mounting
  • Protects fluorescent dyes from rapid photobleaching
  • Compatible with cyanine-based fluorophores like Cy®3, Cy®5, and Alexa Fluor® 647, unlike VECTASHIELD®
  • Available with or without DAPI for nuclear counterstaining
  • Works as well as original EverBrite™, but less viscous for rapid DAPI staining

Drop-n-Stain EverBrite™ Mounting Medium is a wet-set medium that remains liquid. After mounting, coverslips can be sealed with nail polish or Biotium’s CoverGrip™ Coverslip Sealant (23005). Mounted slides can be stored at 4°C or -20°C, protected from light. Antibody-stained slides can be preserved for a year or longer (note: some labels, like phalloidin, are less stable during storage). Drop-n-Stain EverBrite™ Mounting Medium has a refractive index of 1.42.

Drop-n-Stain EverBrite FITC
Photostability of FITC staining in HeLa cells with Drop-n-Stain EverBrite™ Mounting Medium compared to buffer alone.

We also offer EverBrite™ Hardset Mounting Medium, which cures overnight to form a hard seal, eliminating the need to seal the edges of the coverslips.

EverBrite™ Mounting Media

ProductCounterstainCat. No.
EverBrite™ Mounting MediumNone23001
EverBrite™ Mounting Medium with DAPIDAPI23002
Drop-n-Stain EverBrite™ Mounting MediumNone23008
Drop-n-Stain EverBrite™ Mounting Medium with DAPIDAPI23009
EverBrite™ Hardset Mounting MediumNone23003
EverBrite™ Hardset Mounting Medium with DAPIDAPI23004
EverBrite™ Hardset with NucSpot® 640NucSpot® 64023016
EverBrite TrueBlack® Hardset Mounting MediumNone23017
EverBrite TrueBlack® Hardset with DAPIDAPI23018
EverBrite TrueBlack® Hardset with NucSpot® 640NucSpot® 64023019
Alexa Fluor is a registered trademark of Thermo Fisher Scientific; Cy Dye is a registered trademark of GE Healthcare; VECTASHIELD is a registered trademark of Vector Laboratories.
 

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