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Ac-DEVD-AMC

Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.

Product Attributes

CAS number

169332-61-0

Apoptosis/viability marker

Caspase

For live or fixed cells

Cell lysis required

Detection method/readout

Microplate reader (fluorescence)

Assay type/options

Endpoint assay

Substrate specificity

Caspases

Colors

Blue

Excitation/Emission

342/441 nm (end product)

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5 mg
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Product Description

Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.

  • Ex/Em of substrate = 330/390 nm (weak fluorescence)
  • Ex/Em of end product (AMC) = 342/441 nm (strong fluorescence)
  • Off-white solid soluble in DMSO
  • C30H37N5O13
  • MW: 675.74

Biotium Catalog #10202

References

1. Anal Biochem 292, 313 (2001).

2. Biochemistry 39, 16056 (2000).

3. Br J Pharmacol 129, 1673 (2000).

4. J Biol Chem 275, 21094 (2000).

 

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