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Ac-DEVD-R110

A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.

Product Attributes

Apoptosis/viability marker

Caspase

For live or fixed cells

Cell lysis required

Detection method/readout

Microplate reader (fluorescence)

Assay type/options

Endpoint assay

Substrate specificity

Caspases

Colors

Green

Excitation/Emission

496/520 nm

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Technology

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

Ac-DEVD-R110 (Rhodamine 110, bis-(N-Ac-L-aspartyl-L-glutamyl-L-valyl-1-aspartic acid amide)) is a fluorogenic substrate for caspase-3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. Ac-DEVD-R110 contains two DEVD tetrapeptides and is hydrolyzed by caspase-3/7 in two successive steps to release the green fluorescent dye rhodamine 110 (R110).

For caspase-3 kits using Ac-DEVD-R110, please see our Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit (30008), and our Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit (30009). For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.

  • Abs/Em of end product (R110) = 496/520 nm (strong fluorescence)
  • Off-white solid soluble in DMSO
  • C60H70N10O25
  • MW: 1331

References

1. Cell Death Differ 6(2), 99(1999) doi:10.1038/sj.cdd.4400476
2. J Biol Chem 275, 288(2000) doi:10.1074/jbc.M112.447540
3. Biochemistry 38(42), 13906(1999) doi:10.1021/bi9913395

 

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