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Calcein AM Cell Viability Assay Kit

The Calcein AM Cell Viability Assay Kit is designed to quantify live cell numbers based on their endogenous esterase activity and plasma membrane integrity.

Product Attributes

Apoptosis/viability marker

Metabolic activity, Live cell stain

For live or fixed cells

For live/intact cells

Detection method/readout

Microplate reader (fluorescence), Fluorescence microscopy, Live cell imaging, Flow cytometry

Assay type/options

Short term staining (<24h), Endpoint assay

Colors

Green

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Product Description

Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation. Once inside the cells, calcein AM, a nonfluorescent molecule itself, is hydrolyzed by endogenous esterase into the highly negatively charged green fluorescent calcein. Green fluorescent calcein is retained in the cytoplasm in live cells. Calcein AM has served as a useful tool for the studies of cell membrane integrity and for cell tracing. The Calcein AM Cell Viability Assay Kit is designed to quantify live cell numbers based on their endogenous esterase activity and plasma membrane integrity. It is a true end-point assay for cell viability. The fluorescent signal is monitored using 485 nm excitation wavelength and 530 nm emission wavelength. The fluorescent signal generated from the assay is proportional to the number of living cells in the sample.

We also supply Calcein AM in a variety of packaging sizes and formats.

Kit Components:

  • Calcein AM in anhydrous DMSO

Features:

  • True end-point viability assay: Detect only live cells with intact plasma membrane integrity
  • Simple & Fast: 30-min assay

References

1. J Neurosci Methods. 50, 205 (1993).

2. J Immunol Methods 163,181 (1993).

3. Cancer Lett. 87, 199 (1994).

4. J Immunol Methods 178, 41 (1995).

 

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