Support & Resources

+1

Calcein AM Cell Viability Assay Kit

Quantify live cell numbers based on their endogenous esterase activity and plasma membrane integrity using green fluorescence. For microplate reader, microscopy, or flow cytometry.

Product Attributes

Apoptosis/viability marker

Metabolic activity, Live cell stain

For live or fixed cells

For live/intact cells

Detection method/readout

Microplate reader (fluorescence), Fluorescence microscopy, Live cell imaging, Flow cytometry

Assay type/options

Short term staining (<24h), Endpoint assay

Colors

Green

Excitation/Emission

485/530 nm (end product)

Storage Conditions

Store at -10 to -35 °C, Protect from light, Desiccate

Size
Catalog #
price
Qty
1,000 assays
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

The Calcein AM Cell Viability Assay Kit is designed to quantify live cell numbers based on their endogenous esterase activity and plasma membrane integrity.

  • Quantitate live cells using green fluorescence
  • 30-min assay
  • True endpoint viability assay; only live cells retain signal
  • For fluorescence microplate reader, fluorescence microscopy, or flow cytometry
  • Ex/Em 485/530 nm

Calcein AM is a widely used green fluorescent cell marker. Calcein AM is itself non-fluorescent and membrane-permeant, and thus can be introduced into cells via incubation. Once inside the cells, endogenous esterases hydrolyze the compound into the highly negatively charged green fluorescent dye calcein, which is retained in the cytoplasm in live cells. Only viable cells with intact plasma membranes retain fluorescence, making this a true end-point assay for cell viability. The fluorescent signal generated from the assay is proportional to the number of living cells in the sample. Calcein AM is also a useful tool for cell tracing.

We supply Calcein AM in a variety of packaging sizes and formats. See our full line of Cell Viability & Apoptosis Assays.

References

1. Brain Res (2009) 1275, 87-95. doi: 10.1016/j.brainres.2009.04.008
2. J Hepatol (2010) 52(5), 690-697. doi: 10.1016/j.jhep.2009.12.025
3. PLoS ONE (2011) 6(6), e20301. doi:10.1371/journal.pone.0020301
4. PLoS ONE (2012) 7(4), e35007. doi:10.1371/journal.pone.0035007
5. Cancer Biol Ther (2013) 14(4),357-364.DOI: 10.4161/cbt.23623
6. Toxics (2014) 2, 258-275. doi:10.3390/toxics2020258
7. Cell Physiol Biochem (2015) 36, 384-394. DOI: 10.1159/000430257
8. RSC Adv (2015) 5, 47749-47756. https://doi.org/10.1039/C5RA06861D
9. Biomaterials (2018) 179, 60-70. doi:10.1016/j.biomaterials.2018.06.027
10. Sci Rep (2018) 8, 766. https://doi.org/10.1038/s41598-017-17539-z
11. Mol Cancer Ther (2019) 18(6), 1092. DOI: 10.1158/1535-7163.MCT-18-1313
12. Sci Rep (2019) 9, 2147. https://doi.org/10.1038/s41598-019-38590-y

 

You may also like…