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Calcein AM Cell Viability Assay Kit

Quantify live cell numbers based on their endogenous esterase activity and plasma membrane integrity using green fluorescence. For microplate reader, microscopy, or flow cytometry.

Product Attributes

Apoptosis/viability marker

Metabolic activity, Live cell stain

For live or fixed cells

For live/intact cells

Detection method/readout

Microplate reader (fluorescence), Fluorescence microscopy, Live cell imaging, Flow cytometry

Assay type/options

Endpoint assay, Short term staining (<24h)




485/530 nm (end product)

Storage Conditions

Store at -10 to -35 °C, Protect from light, Desiccate

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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Catalog #
1,000 assays
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Product Description

The Calcein AM Cell Viability Assay Kit is designed to quantify live cell numbers based on their endogenous esterase activity and plasma membrane integrity.

  • Quantitate live cells using green fluorescence
  • 30-min assay
  • True endpoint viability assay; only live cells retain signal
  • For fluorescence microplate reader, fluorescence microscopy, or flow cytometry
  • Ex/Em 485/530 nm

Calcein AM is a widely used green fluorescent cell marker. Calcein AM is itself non-fluorescent and membrane-permeant, and thus can be introduced into cells via incubation. Once inside the cells, endogenous esterases hydrolyze the compound into the highly negatively charged green fluorescent dye calcein, which is retained in the cytoplasm in live cells. Only viable cells with intact plasma membranes retain fluorescence, making this a true end-point assay for cell viability. The fluorescent signal generated from the assay is proportional to the number of living cells in the sample. Calcein AM is also a useful tool for cell tracing.

We supply Calcein AM in a variety of packaging sizes and formats. See our full line of Cell Viability & Apoptosis Assays.


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