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Seals the edges of wet-mounted coverslips for fluorescence microscopy, with no ingredients that can leach into aqueous mounting medium and affect specimen fluorescence.
CoverGrip™ Coverslip Sealant is the first product specifically designed for sealing the edges of wet-mounted coverslips for fluorescence microscopy. Unlike nail polish, CoverGrip™ contains no ingredients that can leach into aqueous mounting medium and affect specimen fluorescence.
CoverGrip™ is made with d-limonene, a natural, environmentally-friendly solvent with a pleasant citrus aroma, and dries to form a clear, hard, durable seal along the edges of the coverslip. Available in 15 mL brush-cap applicator bottles or in 100 mL dropper-cap refill bottles. CoverGrip™ is designed to replace nail polish for permanent sealing of wet-mounted coverslips. It can be removed by soaking slides in buffer (see product information sheet for details). However, it is not designed to be a removable temporary seal for hybridization, for example.
EverBrite™ mounting medium does show high background fluorescence with violet excitation. The background is more prominent when imaged using epifluorescence using UV excitation and a DAPI filter cube, but is generally quite low in confocal imaging using 405 nm laser.
CoverGrip™ is designed to replace nail polish for permanent sealing of wet-mounted coverslips. It can be removed by soaking slides in buffer (see the product information sheet for details). CoverGrip™ is not designed to create a removable temporary seal for hybridization.
Yes, EverBrite™ mounting media are compatible with a wide range of dyes. Unlike VectaShield®, EverBrite™ won’t interfere with cyanine-based fluorophores like Alexa Fluor® 647, Cy®3, or Cy®5.
AlexaFluor is a registered trademark of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva; VectaShield is a registered trademark of Vector Laboratories.