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Fluorescence Microscopy
Mounting Media & Microscopy Consumables
CoverGrip™ Coverslip Sealant

CoverGrip™ Coverslip Sealant

Seals the edges of wet-mounted coverslips for fluorescence microscopy, with no ingredients that can leach into aqueous mounting medium and affect specimen fluorescence.

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Store at room temperature

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FAQs

EverBrite™ Mounting Medium & CoverGrip™ Coverslip Sealant
What is the refractive index of EverBrite™ Mounting Medium?

EverBrite™ Mounting Medium (23001) and EverBrite™ Mounting Medium with DAPI (23002) both have a refractive index of 1.46.  EverBrite™ Hardset Mounting Medium (23003) and EverBrite™ Hardset Mounting Medium with DAPI (23004) both have an initial refractive index of 1.38, which increases to 1.42 after 24 hours of curing, and 1.46 after 4 days, after which it remains constant


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Are the EverBrite™ Mounting Media, wetset and hardset, compatible with Alexa Fluor® dyes or Cy® dyes?

Yes, EverBrite™ mounting media are compatible with a wide range of dyes. Unlike VectaShield®, EverBrite™ won’t interfere with cyanine-based fluorophores like Alexa Fluor® 647, Cy®3, or Cy®5.

AlexaFluor is a registered trademark of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva; VectaShield is a registered trademark of Vector Laboratories.


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Can I remove EverBrite™ Mounting Medium from my samples after mounting?

EverBrite™ wet-set mounting medium is an aqueous, glycerol-containing medium. It can be removed from samples by washing several times with PBS or other buffer. However, washing with PBS will not remove DAPI staining.

For coverslips mounted on slides with EverBrite™ Hardset or EverBrite™ wet-set medium, the slides can be soaked in PBS in a slide jar for several hours with gentle agitation until the coverslip slides off (avoid prying or pulling the coverslip, which could damage the sample). Then wash the slide several times to remove any remaining mounting medium. If the coverslips have been sealed with CoverGrip™ Coverslip Sealant, soaking in PBS will remove the sealant as well. Other methods may be required to remove other types of coverslip sealants.


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Product Description

CoverGrip™ Coverslip Sealant is the first product specifically designed for sealing the edges of wet-mounted coverslips for fluorescence microscopy. Unlike nail polish, CoverGrip™  contains no ingredients that can leach into aqueous mounting medium and affect specimen fluorescence.

  • Seals the edges of wet-mounted coverslips for fluorescence microscopy
  • Dries hard and clear
  • Less damaging to samples than nail polish
  • Won’t leach into aqueous mounting medium and affect fluorescence
  • Contains d-limonene

CoverGrip™ is made with d-limonene, a natural, environmentally-friendly solvent with a pleasant citrus aroma, and dries to form a clear, hard, durable seal along the edges of the coverslip. Available in 15 mL brush-cap applicator bottles or in 100 mL dropper-cap refill bottles. CoverGrip™ is designed to replace nail polish for permanent sealing of wet-mounted coverslips. It can be removed by soaking slides in buffer (see product information sheet for details). However, it is not designed to be a removable temporary seal for hybridization, for example.

 

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