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I see high UV/blue background autofluorescence when using EverBrite™ mounting medium. Is this expected? How can it be reduced?

EverBrite™ mounting medium does show high background fluorescence with violet excitation. The background is more prominent when imaged using epifluorescence using UV excitation and a DAPI filter cube, but is generally quite low in confocal imaging using 405 nm laser.


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