Support & Resources

+1

EverBrite™ Hardset Mounting Medium with NucSpot® 640

Biotium’s hardset antifade mounting medium with NucSpot® 640 far-red nuclear counterstain.

Product Attributes

For live or fixed cells

For fixed cells/tissue sections

Excitation/Emission

NucSpot® 640: 649/668 nm (with DNA)

Probe cellular localization

Nucleus

Storage Conditions

Store at 2 to 8 °C, Protect from light

Format
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Size
Catalog #
price
Qty
2 mL
10 mL
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Product Description

This antifade mounting medium features Biotium’s NucSpot® 640 nuclear stain, for one-step mounting and nuclear counterstaining.

Features

  • Self-hardening antifade medium with refractive index well-matched to glass
  • Featuring the only  far-red nuclear counterstains available in mounting media
  • Avoid cross-talk and photoconversion from DAPI
  • Bright and specific nuclear staining with far-red NucSpot® 640

Spectral Properties

  • NucSpot® 640: 649/668 nm (with DNA) (for Cy®5 channel)

EverBrite™ Hardset Mounting Medium cures to form a hard, permanent seal between the slide and coverslip, eliminating the need to seal the coverslip edges. It cures overnight to a refractive index of 1.43, and continues curing over the course of four days to a refractive index of 1.46. It prevents rapid photobleaching of a wide selection of fluorescent dyes. Unlike VECTASHIELD® Mounting Medium, EverBrite™ is compatible with cyanine-based fluorophores and is optimally formulated for use with Biotium’s CF® dyes.

This mounting medium features Biotium’s novel NucSpot® 640 is a far-red dye nuclear counterstain for the Cy®5 channel. Using far-red nuclear stains can avoid problems of fluorescence cross-talk and UV photoconversion with traditional dyes like DAPI.

Note: NucSpot® 640 also shows dim blue fluorescence with the DAPI filter set, and should be tested for suitability before using with blue probes, especially for epifluorescence microscopy.

EverBrite™ Hardset Mounting Medium with near-infrared NucSpot® 680 is coming soon!

See our full selection of antifade mounting media and related products:

ProductNuclear CounterstainCat. No.Features
EverBrite™ Mounting MediumNone23001• Wet-set mounting medium
• Requires coverslip sealing
• Refractive index 1.46
EverBrite™ with DAPIDAPI23002
EverBrite™ with NucSpot® 640 (Coming soon)NucSpot® 640Coming soon
EverBrite™ with NucSpot® 680 (Coming soon)NucSpot® 680Coming soon
Drop-n-Stain EverBrite™ Mounting MediumNone23018• Wet-set mounting medium
• Convenient dropper bottle
• Ideal for wells & chambers
• Refractive index 1.42
Drop-n-Stain EverBrite™ with DAPIDAPI23019
EverBrite™ Hardset Mounting MediumNone23003• Hard-set mounting medium
• Forms hard seal after 24 h
• No coverslip sealing needed
• Refractive index 1.46 after curing
EverBrite™ Hardset with DAPIDAPI23004
EverBrite™ Hardset with NucSpot® 640NucSpot® 64023016
EverBrite™ Hardset with NucSpot® 680 (Coming soon)NucSpot® 680Coming soon
EverBrite TrueBlack® Hardset Mounting MediumNone23017• Unique antifade with lipofuscin quenching
• Quenches as it hardens, with low background
EverBrite TrueBlack® Hardset with DAPIDAPI23018
EverBrite TrueBlack® Hardset with NucSpot® 640NucSpot® 64023019
EverBrite TrueBlack® Hardset with NucSpot® 680 (Coming soon)NucSpot® 680Coming soon
CoverGrip™ Coverslip SealantN/A23005• For sealing edges of wet-set coverslips
* Note: NucSpot® dyes also have dim fluorescence with the DAPI filter set, and should be tested for suitability with blue probes, especially for epifluorescence imaging.
Cy dye is a registered trademark of GE Healthcare; VECTASHIELD is a registered trademark of Vector Laboratories.
 

You may also like…