AccuClear® dsDNA Standard, 25 ng/uL

Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

One exception that we are aware of is GelGreen™, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen™ is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining.

AccuGreen™ kits are designed for use with the Qubit® fluorometer. All of the other kits are designed for use with 96-well fluorescence plate readers. Our quantitation kits vary in the concentration ranges of dsDNA that they are able to detect. Some of the kits also have different fluorescence excitation/emission. Visit the DNA Quantitation Kit Technology Page or consult the table below to make your selection.

All DNA & RNA Quantitation Kits

The AccuGreen™ DNA Quantitation Kits are designed for use on the Qubit® fluorometer. The AccuGreen™ High Sensitivity DNA Quantitation Kit is a direct replacement for the Qubit® dsDNA HS Assay Kit from Thermo Fisher Scientific. The AccuGreen™ Broad Range DNA Quantitation Kit is a replacement for the Qubit® dsDNA BR Assay Kit from Thermo Fisher Scientific.

The AccuBlue® Broad Range RNA Quantitation is optimized for use with the Qubit® fluorometer using the pre-programmed RNA Broad Range program. It also can be used with fluorescence microplate readers equipped for detecting far-red fluorescence (Ex/Em 630/660 nm). The kit has a wide linear range of 5-1000 ng RNA, spanning those of both the Quant-iT™ RNA and Quant-iT™ RNA Broad Range Kits from Thermo Fisher Scientific.

The AccuClear® and AccuBlue® DNA Quantitation Kits are designed for use with fluorescence 96-well plate readers. AccuClear®, AccuBlue® NextGen and AccuBlue® High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor®-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kits, which are designed for use on that instrument.

The AccuBlue® Broad Range Kit requires an instrument equipped to read blue fluorescence (Ex/Em 350/460 nm).

<div> Quant-iT and Qubit are trademarks or registered trademarks of Thermo Fisher Scientific. QuantiFluor is a registered trademark of Promega.</div>