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Oxazole Yellow (YO-PRO®-1), 1 mM in DMSO

Oxazole Yellow, also known as YO-PRO®-1, is a green-fluorescent, cell-impermeant nucleic acid stain that can be used as a early marker of apoptosis.

Product Attributes

Apoptosis/viability marker

Dead cell stain

For live or fixed cells

For fixed cells, For live/intact cells

Detection method/readout

Fluorescence microscopy, Flow cytometry

Probe cellular localization

Nucleus & cytoplasm

Assay type/options

No-wash staining, Real-time imaging

Cell permeability

Membrane impermeant

Colors

Green

CAS number

152068-09-2

Excitation/Emission

491/506 nm (with DNA)

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Product Description

Oxazole Yellow, also known as YO-PRO®-1, is a green-fluorescent, cell-impermeant, high-affinity carbocyanine monomeric nucleic acid stain. It is essentially non-fluorescent in the absence of nucleic acids but exhibits excitation/emission maxima 491/506 nm when bound to nucleic acids. Oxazole Yellow is also used to identify apoptotic cells. Early apoptotic cells become permeant to Oxazole Yellow, but remain impermeant to propidium iodide (cat. no. 40016), a dead cell stain.

  • Monomeric nucleic acid stain
  • Cell-impermeant
  • Early marker of apoptosis
  • λExEm (with DNA) = 491/506 nm
  • Supplied at 1 mM in DMSO
  • C24H29I2N3O
  • MW: 629

See the table below for other chemical equivalents of Thermo Fisher Scientific’s branded dead-cell selective nucleic acid dyes.

Product

Equivalent to

Color (Ex/Em)

Catalog No.

Oxazole Blue, 1 mM in DMSOPO-PRO™-1Blue (434/457 nm) 40091
Oxazole Blue Homodimer, 1 mM in DMSOPOPO™-1Blue (433/457 nm) 40093
Oxazole Yellow, 1 mM in DMSOYO-PRO®-1Green (491/506 nm) 40089
Oxazole Yellow Homodimer, 1 mM in DMSOYOYO®-1Green (491/508 nm) 40090
TO Iodide, 1 mM in DMSOTO-PRO®-1Green (515/531 nm)40088
Thiazole Orange Homodimer, 1 mM in DMSOTOTO®-1Green (514/531 nm)40079
Oxazole Red, 1 mM in DMSOYO-PRO®-3Far-red (613/629 nm)40105
Oxazole Red Homodimer, 1 mM in DMSOYOYO®-3Far-red (612/631 nm)40106
Thiazole Red, 1 mM in DMSOTO-PRO®-3Far-red (642/657 nm)40087
Thiazole Red Homodimer, 1 mM in DMSOTOTO®-3Far-red (642/661 nm)40080
YOYO, YO-PRO, POPO, PO-PRO, TOTO, and TO-PRO are trademarks and registered trademarks of Thermo Fisher Scientific.
 

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