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Mag-Fluo-4, AM Ester

Membrane-permeant AM ester form of Mag-Fluo-4, a fluorescent magnesium and calcium ion indicator that is an analog of Fluo-4.

Product Attributes

Applications

Calcium indicators, Magnesium indicators

Cell permeability

Membrane permeant

Indicator type

Non-ratiometric

Excitation/Emission

493/517 nm (high Mg2+ or Ca2+)

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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10 x 50 ug
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Product Description

Membrane-permeant AM ester form of Mag-Fluo-4, a fluorescent ion indicator and analog of Fluo-4. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic® F-127 (cat# 59004) is often used as a dispersing agent to facilitate loading. The indicator has an affinity for magnesium (Kd = 4.7 mM) and low-affinity for calcium (Kd = 22 uM) with absorbance/emission at 493/517 nm (high Mg2+).

    • λExEm (high Mg2+ or Ca2+) (after hydrolysis): 493/517 nm
    • Orange solid soluble in DMSO
    • Store -20°C, desiccate and protect from light
    • C37H33F2NO18
    • MW: 818


 
 
BAPTA-based ion indicators like Mag-Fluo-4 have been shown to be fixable in situ by EDC/EDAC (cat# 59002). The fixation of indicator dyes is useful for downstream immunofluorescence and IHC studies (Cell Calcium 1997, 21(3), 175).
 

Pluronic is a registered trademark of BASF.

References

1. Biophys J. (2009) Oct 7; 97(7):1864 doi: 10.1016/j.bpj.2009.07.021
2. Biochem J. (2002) Oct 1; 367(Pt1):137 doi: 10.1042/BJ20020305
3. Biochima et Biophys Acta - Mol Cell Res. (2017) June; 1864(6):977 doi.org/10.1016/j.bbamcr.2016.11.026
4. Biochem & Biophys Res Comm. (2014) November 28; 454(4):572 doi.org/10.1016/j.bbrc.2014.10.125

 

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