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DNAzure® 2.0 Visible Blue DNA Gel Stain Kit

A blue nucleic acid gel stain to visualize dsDNA in agarose or polyacrylamide gels by the unaided eye. Developed with improved sensitivity compared to the original DNAzure® Blue Nucleic Acid Gel Stain,

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Stains 20 gels
Stains 4 gels
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DNAzure® 2.0 Visible Blue DNA Gel Stain Kit
DNAzure® 2.0 Visible Blue DNA Gel Stain Kit - Image 2Time course of DNAzure® 2.0 visible color development by light exposure. Biotium's 1 kb DNA ladder in the amounts shown was separated on a 1% agarose/TBE gel and stained with DNAzure® 2.0. The gel was exposed on the Glo-Plate™ White Photoactivation Device for the times indicated and photographed at each time point. The 500 bp band boxed in red contains 0.6 ng DNA.
DNAzure® 2.0 Visible Blue DNA Gel Stain Kit - Image 3The DNAzure® 2.0 Visible Blue DNA Gel Stain Kit produces stronger signal and higher sensitivity than original DNAzure® Blue stain. Duplicate dilution series of 1 kb DNA ladder in the amounts shown per lane were separated on a 1% agarose/TBE gel. Half the gel was stained with DNAzure® 2.0 (left) and the other half was stained with original DNAzure® (right). Color was developed for 60 minutes on the Glo-Plate™ White Photoactivation Device. The gels were scanned on a LICORbio® Odyssey® M imaging system in the 630 trans (visible color) channel. The 500 bp band boxed in red contains 0.3 ng DNA.
DNAzure® 2.0 Visible Blue DNA Gel Stain Kit - Image 4DNA PAGE gel staining with DNAzure® 2.0. A titration series of Low Molecular Weight DNA Ladder (NEB) was separated on a 10% acrylamide/1X TBE minigel. The gel was stained in DNAzure® 2.0, exposeed to Glo-Plate™ White Photoactivation Device for 15 minutes to develop the visible color, and photographed. The same gel was stored for one week in water at room temperature before scanning on a LICORbio® Odyssey M imaging system in the 630 trans channel for visible color and the 700 channel for near-IR fluorescence. The boxed bands contain 1 ng DNA for the 350 bp band and 3 ng DNA for the 50 bp band.

Product Description

The DNAzure® 2.0 Visible Blue DNA Gel Stain Kit lets you visualize DNA bands with the naked eye with no need for a UV light source. DNAzure® 2.0 delivers excellent sensitivity, detecting as little as ~1 ng of double-stranded DNA in both agarose and polyacrylamide gels.

Features

  • Deep blue bands visible by the naked eye following light exposure
  • Ultrasensitive detection of dsDNA (as little as ~1 ng DNA) in agarose or acrylamide gels
  • Simplified DNA band excision without DNA-damaging UV light
  • Stained bands are stable in gel for weeks
  • Near-IR fluorescence (~700 nm) detectable on LICORbio® Odyssey®
  • Stronger signal and higher sensitivity compared to original DNAzure® Stain

Kit components

  • Component A, 100X
  • Component B, 100X
  • Component C, 10X
  • 6X DNA Loading Buffer (Orange)

DNAzure® 2.0 Visible Blue DNA Gel Stain Kit is a new and improved formulation of our original DNAzure® Blue Nucleic Acid Gel Stain, delivering enhanced sensitivity for visible staining of double-stranded DNA in both agarose and polyacrylamide gels.

DNAzure® 2.0 utilizes a DNA-binding dye that transforms from colorless to deep blue upon exposure to bright light, with sensitivity comparable to the most sensitive fluorescent stains and the ability to detect less than 1 ng of DNA per band. After light exposure, bands are visible to the naked eye with no need for a UV transilluminator or LED light box. Stained gels remain stable for weeks after color development, making it easy to revisit results on your timeline.

DNAzure® 2.0 also has near-infrared (near-IR) fluorescence at ~700 nm, detectable with the LICORbio® Odyssey® and similar near-IR imaging systems. Detection sensitivity is comparable for both visible color and near-IR imaging. The vivid blue staining makes it simple to identify and cut out bands for gel purification, and DNA is fully compatible with downstream applications such as sequencing and cloning after dye removal using a standard gel extraction kit.

This kit is supplied with 6X DNA Loading Buffer (Orange) for preparing your samples for electrophoresis. The orange tracking dye migrates at approximately 50 bp in a 1% agarose gel and will not mask the color of DNAzure®-stained bands.

For best results, pair DNAzure® 2.0 with the Glo-Plate™ White Photoactivation Device, an affordable light panel that provides fast, even activation for gel development.

DNAzure® 2.0 contains a double-stranded DNA dye, and will not stain RNA or single-stranded DNA. We recommend our EMBER™ Ultra DNA or RNA Gel Staining Kits (Cat. No. 41043, 41044) for ultrasensitive detection of DNA or RNA in agarose gels. Oxazole Gold (Cat. No. 40094) is recommended for high-sensitivity staining of single-stranded nucleic acids in acrylamide gels.

New Formulation with Enhanced Sensitivity Compared to Original DNAzure®

The DNAzure® 2.0 Visible Blue DNA Gel Stain Kit produces stronger signal and higher sensitivity than original DNAzure® Blue stain. Duplicate dilution series of 1 kb DNA ladder in the amounts shown per lane were separated on a 1% agarose/TBE gel. Half the gel was stained with DNAzure® 2.0 (left) and the other half was stained with original DNAzure® (right). Color was developed for 60 minutes on the Glo-Plate™ White Photoactivation Device. The gels were scanned on a LICORbio® Odyssey® M imaging system in the 630 trans (visible color) channel. The 500 bp band boxed in red contains 0.3 ng DNA.

Detect By Visible Blue Bands or Near-IR Fluorescence

DNA PAGE gel staining with DNAzure® 2.0. A titration series of Low Molecular Weight DNA Ladder (NEB) was separated on a 10% acrylamide/1X TBE minigel. The gel was stained in DNAzure® 2.0, exposeed to Glo-Plate™ White Photoactivation Device for 15 minutes to develop the visible color, and photographed. The same gel was stored for one week in water at room temperature before scanning on a LICORbio® Odyssey M imaging system in the 630 trans channel for visible color and the 700 channel for near-IR fluorescence. The boxed bands contain 1 ng DNA for the 350 bp band and 3 ng DNA for the 50 bp band.

 

Choose the Right Stain for Your Application

Learn about the Original GelRed® Nucleic Acid Gel Stain or GelGreen® Nucleic Acid Gel Stain. GelRed® 3X in water is ready-to-use for post-electrophoresis gel staining, and is supplied in a 4L Cubitainer®. Higher concentrations of Original GelRed® are available as 10,000X in water or DMSO. We also offer GelRed® Agarose and GelRed® Prestain Plus 6X Loading Dye. GelGreen® Nucleic Acid Gel Stain is a safer replacement for SYBR® gel stains and is compatible with visible light excitation. For ultrasensitive detection  of DNA or RNA in agarose gels, we recommend our EMBER™ Ultra DNA or RNA Gel Staining Kits (Cat. No. 41043, 41044). Our Go-Go™ Fast DNA Gel Running Buffer allows running gels 3X faster than with TAE or TBE buffer.

Product / MethodProcedureAdvantagesDisadvantagesRecommended for
DNA staining with EMBER™ Ultra DNA Gel KitAgarose is supplied pre-coated with EMBER™ Ultra Dye, just dissolve, heat, and pour.• Safer and more convenient, no need to handle concentrated dye

• Superior sensitivity, detect as little as ≤1 ng DNA

• No need for post-electrophoresis staining

• Optimal for blue LED gel imagers		• Not suitable for PAGE, DGGE, EMSA, or PFGE gels

• Dye may cause band migration issues when loading larger amounts of DNA (more than ~200 ng/band), or for some restriction digests		• Routine agarose gels
RNA staining with EMBER™ Ultra RNA Gel KitAgarose is supplied pre-coated with EMBER™ Ultra Dye, just dissolve, heat, and pour.• Safer and more convenient stain for RNA, no need to handle concentrated dye

• Superior sensitivity, detect as little as ≤5 ng RNA

• No need for post-electrophoresis staining

• Included loading dye contains formamide for denaturing

• Optimal for blue LED gel imagers		• Will stain DNA as well as RNA

• Dye may cause band migration issues when loading larger amounts of RNA (more than ~200 ng/band)		• Routine RNA gel electrophoresis

• Evaluate total RNA integrity and DNA contamination
DNA prestaining with GelRed® Prestain Plus 6X DNA Loading DyeGelRed® loading buffer is added directly to the DNA sample before loading• Fast & simple: one-step sample loading & DNA staining

• Less concentrated dye for safer handling

• Can re-run a gel to use empty lanes
• Not recommended for PAGE, DGGE, EMSA, or PFGE gels

• Dye may cause band migration issues when loading larger amounts of DNA (more than ~100 ng/band), or for some restriction digests		• Routine agarose gels

• Recommended loading 50-200 ng ladder or 2-5 uL PCR product ( ~100 ng/band or less)
Precast staining with GelRed® 10,000X in water or GelGreen® 10,000X in water

GelRed® or GelGreen® is mixed with molten agarose before gel castingFamiliar protocol, rapid results
Precast staining with GelRed® Agarose LE or GelGreen® Agarose LE
Agarose is supplied pre-coated with GelRed® or GelGreen®, just dissolve, heat, and pourSafer & more convenient, no need to handle concentrated dye
Post-electrophoresis staining with GelRed® 10,000X in water or GelGreen® 10,000X in water
- or -
GelRed® 3X in water
No fluorescent dye is added to the gel, it is stained in 3X GelRed® or 3X GelGreen® solution after electrophoresis• Most accurate sizing/sharpest bands

• Staining solution can be re-used

• Enhance sensitivity by adding NaCl		Extra staining step (up to 30 minutes) after electrophoresis (some customers report good results after only 5 minutes if dye is not reused)• Highly accurate band sizing

• Gels with more than ~100 ng DNA per band

• Analyzing restriction digests
Post-electrophoresis staining with DNAzure® 2.0 Visible Blue DNA Gel Stain KitNo fluorescent dye is added to the gel, it is stained in DNazure® 2.0 solution and then exposed to a bright light source to generate visible blue DNA bands.

We recommend the Glo-Plate™ White Photoactivation Device as a light source for developing DNAzure® 2.0-stained gels		• Allows visualization of DNA bands by the naked eye, no need for a UV light source

• Detect as little as ~1 ng DNA

• Stained bands are stable in gel for weeks

• Also emits near-IR fluorescence (~700 nm) for detection on near-IR imaging systems		Extra staining step (up to 30 minutes) followed by a light exposure step (up to 30 minutes) to generate visible blue DNA bands

• Routine DNA agarose gels

• Visualizing gels without the UV light or expensive imaging systems

• Recommended loading 50-200 ng DNA per lane
Post-electrophoresis staining of PAGE gels using PAGE GelRed® 10,000X or 1X in waterNo fluorescent dye is added to the gel, it is stained in 1X PAGE GelRed® solution after electrophoresis• Formulated for efficient penetration and staining of polyacrylamide gels

• Like the classic GelRed®, it is safe and environmentally friendly		Extra staining step of approx. 30 minutes after electrophoresisStaining of nucleic acids in PAGE gels
DNAzure is a registered trademark of Biotium, Inc. LICORbio and Odyssey are registered trademarks of LI-COR Inc.

Product Attributes

Size
Stains 20 gels, Stains 4 gels
DNA/RNA dye
DNA dye
Storage Conditions
Store at 2 to 8 °C, Protect from light
Assay type/options
DNA gel staining

Documents, Protocols, SDS and COA

FAQs

DNAzure® Visible DNA Gel Stain

We have tested a variety of different light sources for the development of DNAzure® bands in an agarose gel. We have found that most light sources will work, but how long the development takes is dependent on the brightness of the light. The fastest band development is seen with our Glo-Plate™ White Photoactivation Device and Glo-Plate™ 2.0 Blue LED Illuminator. We have also seen good results with other bright, white LED lights. Other light sources that work but take more time include an LED desk lamp and a cell phone light. We found that a 600W halogen lamp did not work well- it was too hot, which melted the gel.

We recommend storing DNAzure® at 4°C. After storage at room temperature, we have seen some loss of dye stability.

Yes, the 1X DNAzure® staining solution can be re-used for multiple gels, under certain conditions. Importantly, the staining solution must be removed from the gel before the gel is exposed to light to develop the bands. If the staining solution undergoes the light exposure, it will not be able to stain another gel. The used staining solution should be stored in the refrigerator, protected from light, between uses. We have successfully re-used the staining solution stored for up to 2 weeks, and used up to 4 times with little loss of signal (after 5 or 6 uses, the sensitivity was noticeably lower).

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