- 6X loading dye includes GelRed® DNA stain
- Combine gel loading and DNA staining in one step
- Improved formulation to minimize DNA migration issues
- Two blue tracking dyes that run at ~1.5 kb and ~200 bp in 1% agarose
- Highly sensitive, non-mutagenic DNA detection
GelRed® is a sensitive, stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr). GelRed® Prestain Plus 6X DNA Loading Dye contains density agents, tracking dyes, and GelRed® dye. The 6X prestain loading dye is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting or after electrophoresis. The loading dye contains two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel.
This product is an improved version of our original 6X GelRed® Prestain Loading Buffer (catalog number 41009) with brighter signal and more consistent DNA migration. When DNA is bound to GelRed® before electrophoresis, the ratio of dye to DNA can cause variable shifts in DNA migration, making it difficult to compare DNA fragment sizes between samples. GelRed® Prestain Plus 6X DNA Loading Dye is formulated to minimize this DNA migration shift, for greater consistency. GelRed® prestaining is simple and can avoid migration issues seen with GelRed® precast gels.
GelRed® and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed® without changing your existing imaging system. In addition, GelRed® is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed® is compatible with downstream applications such as sequencing and cloning. It is efficiently removed from DNA by gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.
GelRed® was subjected to a series of tests at Biotium and by three independent testing services to assess the dye’s safety for routine handling and disposal. Test results confirm that the dye is impenetrable to both latex gloves and cell membranes. The dye is noncytotoxic, nonmutagenic, and classified as non-hazardous for disposal under CCR Title 22 Hazardous Waste Characterization. See the GelRed® and GelGreen® Safety Report.
Agarose Gel Staining Methods Comparison
|Product / Method||Procedure||Advantages||Disadvantages||Recommended for
|DNA prestaining with GelRed® Prestain Plus 6X DNA Loading Dye||GelRed® loading buffer is added directly to the DNA sample before loading||• Fast & simple: one-step sample loading & DNA staining|
• Less concentrated dye for safer handling
• Can re-run a gel to use empty lanes
|• Not recommended for PAGE, DGGE, EMSA, or PFGE gels|
• Dye may cause band migration issues when loading larger amounts of DNA (more than ~100 ng/band), or for some restriction digests
|• Routine agarose gels
• Recommended loading 50-200 ng ladder or 2-5 uL PCR product ( ~100 ng/band or less)
|Precast gel staining with GelRed® 10,000X in water||GelRed® is mixed with molten agarose before gel casting||Familiar protocol, rapid results
|Precast gel staining with GelRed® Agarose LE|
|Agarose is supplied pre-coated with GelRed®, just dissolve, heat, and pour||Safer & more convenient, no need to handle concentrated dye
|Post-electrophoresis gel staining with GelRed® 10,000X in water|
- or -
GelRed® 3X in water
|No fluorescent dye is added to the gel, it is stained in 3X GelRed® solution after electrophoresis||• Most accurate sizing/sharpest bands |
• Staining solution can be re-used
• Enhance sensitivity by adding NaCl
|Extra staining step (up to 30 minutes) after electrophoresis (some customers report good results after only 5 minutes if dye is not reused)||• Highly accurate band sizing
• If more than ~100 ng DNA per band must be loaded
• Analyzing restriction digests