Can the 1X DNAzure® staining solution be re-used?

The 1X DNAzure® staining solution from the original DNAzure® Blue Nucleic Acid Gel Stain can be re-used for multiple gels, under certain conditions. Importantly, the staining solution must be removed from the gel before the gel is exposed to light to develop the bands. If the staining solution undergoes the light exposure, it will not be able to stain another gel. The used staining solution should be stored in the refrigerator, protected from light, between uses. We have successfully re-used the staining solution stored for up to 2 weeks, and used up to 4 times with little loss of signal (after 5 or 6 uses, the sensitivity was noticeably lower).

We do not recommend reusing or storing the 1X staining solution from the DNAzure® 2.0 Visible Blue DNA Gel Stain Kit. The 1X staining solution should be prepared on the day of use.