ExoBrite™ IgG1 Isotype Control Flow Antibody has been found to not react with any target in human cells and have the same isotype as the ExoBrite™ Flow Antibodies against tetraspanin proteins CD9, CD63, and CD81. The antibody is available with the same conjugates as ExoBrite™ Flow Antibodies for the Pacific Blue™, FITC, and PE channels.
- Non-reactive IgG1 mouse antibody
- Serves as a isotype negative control for ExoBrite™ Flow Antibodies
- Available in 3 colors
Extracellular vesicles (EVs), including exosomes, are lipid-bound vesicles that are released from cells. EVs display specific surface proteins and can carry nucleic acids and other cargo, allowing them to transfer biological information between cells in different parts of the body. Therefore exosomes are increasingly studied for their potential use in drug delivery and medical diagnostic applications. The most common proteins used as exosome markers are CD9, CD63, and CD81, members of the tetraspanin family. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of exosomes, but their expression levels vary depending on the cell type of origin.
Exosome antibodies you can trust
Other commercially available antibodies for tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated exosomes and may require tedious optimization for your exosome prep and staining protocol. ExoBrite™ Flow Antibody Conjugates were validated to offer bright signal and low background of exosome markers in purified and bead-bound exosomes.
Biotium also offers ExoBrite™ EV Membrane Staining Kits for detection of isolated EVs and exosomes by flow cytometry. ExoBrite™ EV Membrane Staining Kits may also be combined with antibody staining, for multi-parameter analysis.
ExoBrite™ Flow Antibody Conjugates