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ExoBrite™ CD63 (Mouse) Flow Antibody

Validated antibody for optimal detection of mouse CD63 EV marker in purified or bead-bound EVs by flow cytometry.

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Product Description

ExoBrite™ CD63 (Mouse) Flow Antibody is validated by Biotium for detection of mouse CD63 EV marker in isolated EVs. The antibody conjugates offer exceptional signal-to-noise and include color options for the Pacific Blue™, FITC, PE, and APC channels.

  • NEW: Now with updated buffer formulation for improved signal-to-noise
  • Optimal detection of mouse CD63 EV marker by flow cytometry
  • Validated for purified or bead-bound EVs
  • Bright signal and low background
  • Available in 4 colors

The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of EVs, but their expression levels vary depending on the cell type of origin.

EV antibodies you can trust

Other commercially available antibodies for tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated EVs and may require tedious optimization for your EV prep and staining protocol. The antibodies and dye labels of ExoBrite™ Flow Antibody Conjugates were carefully selected and validated for robust detection of isolated EVs. In addition, the antibodies are provided in a proprietary buffer formulation for reduced antibody aggregation and brighter EV staining for optimal accuracy and signal-to-noise.

For general EV staining, Biotium’s ExoBrite™ True EV Membrane Stains offer unparalleled coverage of EVs in a sample and address issues of dye aggregation often seen with PKH and other common membrane dyes. Biotium also offers optimized ExoBrite™ EV Surface stains conjugated to cholera toxin B (CTB)wheat germ agglutinin (WGA), and Annexin V. These stains are specially formulated for bright and specific detection of isolated EVs by flow cytometry. These ExoBrite™ EV Surface stains may also be combined with antibody staining, for multi-parameter analysis.

Biotium also offers conjugated ExoBrite™ Western Antibodies against CD9CD63, and CD81 designed for optimal detection in EV extracts by fluorescent western blot.

Note: In our testing, we have found that ExoBrite™ 490/515 dye may bind to streptavidin coated surfaces or beads if free biotin binding sites are not blocked. We recommend performing a biotin blocking step after binding your biotinylated capture antibody to streptavidin beads or surfaces when using ExoBrite™ 490/515 conjugates. Alternatively, consider using a different ExoBrite™ dye for staining EVs captured on streptavidin beads or surfaces.

ExoBrite™ Flow Antibody Conjugates

AntibodyEx/EmTargetSpecies ReactivityDetection ChannelCatalog No.
ExoBrite™ 410/450
CD9 Flow Antibody
416/452 nmCD9HumanPacific Blue ™P003-410
ExoBrite™ 490/515
CD9 Flow Antibody
490/516 nmCD9HumanFITCP003-490
ExoBrite™ 560/585
CD9 Flow Antibody
562/584 nmCD9HumanPEP003-560
ExoBrite™ 640/660
CD9 Flow Antibody
642/663 nmCD9HumanAPCP003-640
ExoBrite™ R-PE
CD9 Flow Antibody
496, 546, 565/578 nmCD9HumanPEP003-RPE
ExoBrite™ APC
CD9 Flow Antibody
651/660 nmCD9HumanAPCP003-APC
ExoBrite™ 410/450
CD9 (Mouse) Flow Antibody
416/452 nmCD9MousePacific Blue ™P018-410
ExoBrite™ 490/515
CD9 (Mouse) Flow Antibody
490/516 nmCD9MouseFITCP018-490
ExoBrite™ 560/585
CD9 (Mouse) Flow Antibody
562/584 nmCD9MousePEP018-560
ExoBrite™ 640/660
CD9 (Mouse) Flow Antibody
642/663 nmCD9MouseAPCP018-640
ExoBrite™ 410/450
CD63 Flow Antibody
416/452 nmCD63
HumanPacific Blue ™P004-410
ExoBrite™ 490/515
CD63 Flow Antibody
490/516 nmCD63
HumanFITCP004-490
ExoBrite™ 560/585
CD63 Flow Antibody
562/584 nmCD63
HumanPEP004-560
ExoBrite™ 640/660
CD63 Flow Antibody
642/663 nmCD63
HumanAPCP004-640
ExoBrite™ R-PE
CD63 Flow Antibody
496, 546, 565/578CD63
HumanPEP004-RPE
ExoBrite™ APC
CD63 Flow Antibody
651/660 nmCD63
HumanAPCP004-APC
ExoBrite™ 410/450
CD63 (Mouse) Flow Antibody
416/452 nmCD63
MousePacific Blue ™P022-410
ExoBrite™ 490/515
CD63 (Mouse) Flow Antibody
490/516 nmCD63
MouseFITCP022-490
ExoBrite™ 560/585
CD63 (Mouse) Flow Antibody
562/584 nmCD63
MousePEP022-560
ExoBrite™ 640/660
CD63 (Mouse) Flow Antibody
642/663 nmCD63
MouseAPCP022-640
ExoBrite™ 410/450
CD81 Flow Antibody
416/452 nmCD81HumanPacific Blue ™P005-410
ExoBrite™ 490/515
CD81 Flow Antibody
490/516 nmCD81HumanFITCP005-490
ExoBrite™ 560/585
CD81 Flow Antibody
562/584 nmCD81HumanPEP005-560
ExoBrite™ 640/660
CD81 Flow Antibody
642/663 nmCD81HumanAPCP005-640
ExoBrite™ R-PE
CD81 Flow Antibody
496, 546, 565/578CD81HumanPEP005-RPE
ExoBrite™ APC
CD81 Flow Antibody
651/660 nmCD81HumanAPCP005-APC
ExoBrite™ 410/450
CD81 (Mouse/Rat) Flow Antibody
416/452 nmCD81Mouse, RatPacific Blue ™P019-410
ExoBrite™ 490/515
CD81 (Mouse/Rat) Flow Antibody
490/516 nmCD81Mouse, RatFITCP019-490
ExoBrite™ 560/585
CD81 (Mouse/Rat) Flow Antibody
562/584 nmCD81Mouse, RatPEP019-560
ExoBrite™ 640/660
CD81 (Mouse/Rat) Flow Antibody
642/663 nmCD81Mouse, RatAPCP019-640
ExoBrite™ 410/450 IgG1
Isotype Control Flow Antibody
416/452 nmIsotype ControlNonePacific Blue ™P008-410
ExoBrite™ 490/515 IgG1
Isotype Control Flow Antibody
490/516 nmIsotype ControlNoneFITCP008-490
ExoBrite™ 560/585 IgG1
Isotype Control Flow Antibody
562/584 nmIsotype ControlNonePEP008-560
ExoBrite™ R-PE IgG1
Isotype Control Flow Antibody
496, 546, 565/578Isotype ControlNonePEP008-RPE
ExoBrite™ 640/660 IgG1
Isotype Control Flow Antibody

642/663 nmIsotype ControlNoneAPCP008-640
 

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