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Blood Group B Monoclonal Mouse Antibody (HEB-29)

The antibody HEB-29 reacts with human blood group B.

Product Attributes

Antibody number

1065

Reactivity (target)

Blood Group B

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

HEB-29

Isotype

IgM, kappa

Molecular weight

Multiple

Human gene symbol

ABO

Entrez gene ID

28

SwissProt

P16442

Unigene

654423

Immunogen

Mixture of erythrocytes of group B and glycoprotein fraction isolated from saliva of secretors with blood group B

Cellular localization

Membrane/cell surface

Species reactivity

Human

Applications

Agglutination, Immunofluorescence, Immunohistology (formalin)

Application notes

Agglutination, Immunohistology (formalin), Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

KG1 cells or human colorectal carcinoma tissues.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Cancer, Hematology

Conjugation
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Product Description

The antibody HEB-29 reacts with human blood group B. The specificity of the antibody HEB-29 was confirmed by comparison of specificity and reactivity to standard reagent using >5.000 samples of blood. MAb HEB-29 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. It is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

References

Vanak J, et al. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma. 1989;36(4):479-88

 

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