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Vimentin Monoclonal Mouse Antibody (LN-6)

This MAb reacts with a 58 kDa protein identified as vimentin.

Product Attributes

Antibody number

0060

Reactivity (target)

Vimentin

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

LN-6

Isotype

IgM

Molecular weight

57-60 kDa

Synonyms

VIM

Human gene symbol

VIM

Entrez gene ID

7431

SwissProt

P08670

Unigene

455493

Immunogen

Human thymic nuclear extract

Cellular localization

Cytoskeleton

Species reactivity

Cat, Cow, Human, Mouse, Pig, Rabbit, Rat, Sheep

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology formalin-fixed 1-2 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

Jurkat cells, Sarcomas, Melanomas

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Cancer, Cytoskeleton

Conjugation
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Product Description

This MAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP’s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

References

Stathopoulos, E., et al., J. Histochem. Cytochem. 37, 1363 (1989)

 

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