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NucView® 488 Caspase-3 Enzyme Substrate, 1 mM in DMSO

NucView® 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time.

Product Attributes

Apoptosis/viability marker

Caspase

For live or fixed cells

For live/intact cells

Assay type/options

Long term staining (24-72h), Endpoint assay, No-wash staining, Real-time imaging, Homogenous assay

Detection method/readout

Fluorescence microscopy, Live cell imaging, Flow cytometry

Substrate specificity

Caspases

Colors

Green

Fixation options

Fix after staining (formaldehyde), Permeabilize after staining

Excitation/Emission

500/530 nm (with DNA)

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10 uL
100 uL
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Product Description

NucView® 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time. Visit our Product Video page to see a video of NucView® 488 in action.

Unlike conventional caspase assays, NucView® 488 Caspase-3 Substrate detects caspase-3/7 activity within individual intact cells without inhibiting caspase activity. The substrate consists of a fluorogenic DNA dye and a DEVD substrate moiety specific for caspase-3/7. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the NucView® 488 Caspase-3 Substrate is bi-functional, allowing detection of intracellular caspase-3/7 activity and visualization of changes in nuclear morphology during apoptosis. The fluorescent staining produced in response to caspase-3/7 activity is compatible with subsequent fixation and permeabilization for immunostaining.

NucView® 488 Caspase-3 Substrate, is also offered as a 1 mM solution in PBS (phosphate-buffered saline) (catalog no. 10403) for use in cells that are sensitive to DMSO toxicity. In non-DMSO sensitive cell types, adding DMSO during the substrate incubation may enhance NucView® 488 staining. Biotium also offers the blue fluorescent NucView® 405 Caspase-3 Substrate for use with the 405 nm laser line and the orange fluorescent NucView® 530 for detection in the Cy3® channel.

Download the NucView® Flyer for more information.

NucView® enzyme substrate technology is covered by U.S. patents.

References

 

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