Support & Resources

+1

Apoptosis and Necrosis Quantitation Kit Plus

A convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.

Product Attributes

Apoptosis/viability marker

Phosphatidylserine/Annexin V, Dead cell stain, Apoptosis/necrosis assay

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay

Colors

Green/Red

Product origin

Annexin V (human); recombinant, produced in E. coli

Storage Conditions

Store at 2 to 8 °C, Do not freeze, Protect from light

Learn more about

Technology

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Size
Catalog #
price
Qty
50 assays
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

The Apoptosis and Necrosis  Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.

Features

  • Simultaneous detection of apoptotic & necrotic cells
  • 15-30 minute staining
  • Next-generation CF®488A is brighter and more photostable than FITC
  • EthD-III is a superior alternative to PI and EthD-I

Kit Components

  • CF®488A Annexin V (human; recombinant, produced in E. coli)
  • Ethidium Homodimer III (EthD-III)
  • 5X Annexin Binding Buffer

Spectral Properties

  • CF®488A Annexin V: Ex/Em 490/515 nm
  • EthD-III: Ex/Em 279, 532/625 nm (with DNA)

Apoptosis and necrosis are two major processes by which cells die. Apoptosis is an active, genetically regulated disassembly of the cell from within. During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane for phagocytic cell recognition. The human anticoagulant Annexin V is a 35 kDa, Ca2+-dependent phospholipid-binding protein with high affinity for PS. Annexin V labeled with CF®488A stains apoptotic cells green by binding to PS exposed on the cell surface. CF®488A is spectrally similar to fluorescein (FITC), with much brighter and more photostable fluorescence.

Necrosis normally results from severe cellular insult. Both internal organelle and plasma membrane integrity are lost, resulting in the release of cellular contents into the surrounding environment. Ethidium Homodimer III (EthD-III) is a highly positively charged nucleic acid probe, which is impermeant to live cells, but stains cells with compromised membrane integrity with red fluorescence. EthD-III is a superior alternative to Propidium Iodide (PI) or Ethidium Homodimer I because of its significantly higher affinity for DNA and higher fluorescence quantum yield. The Apoptosis & Necrosis Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.

See our full selection of Cell Viability & Apoptosis Assays and Viability & Apoptosis Assays for Flow Cytometry.

 

CF dye technology is covered by US and international patents.

References

1. Biol Reprod (2016) 94(3):69. doi:10.1095/biolreprod.115.134924
2. Toxicol Sci (2016) 154(2): 332–340. doi: 10.1093/toxsci/kfw166
3. J. Mater. Chem. B (2018) 6, 1095-1104. https://doi.org/10.1039/C7TB03261G
4. J Cell Mol Med (2019) 23:7859–7872. DOI: 10.1111/jcmm.14665

 

You may also like…