Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

NucView 488 and MitoView 633 Apoptosis Assay Kit

This kit contains the NucView™488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

Source description: Exposure: 000 : 00 : 00 . 274 : 264

Binning: 2 x 2

Gain: 1.000000

%Accumulated%=0
Catalog #

30062

Size

100 assays

Size
Catalog #
price
Qty
100 assays
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping & Tax (CA only) not included in price. Learn more

Taxes: Tax is assessed in the state of California and is calculated during checkout based on your shipping information. Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

This kit contains the NucView™488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry. The bi-functional NucView™488 Caspase-3 Substrate is able to detect both intracellular caspase-3 and at the same time stain the cell nucleus, which is known to undergo morphological changes during apoptosis. MitoView™633 is a far-red fluorescent mitochondrial dye. The staining is dependent upon the mitochondrial membrane potential; thus, apoptotic cells exhibit a much lower MitoView™633 dye staining compared to healthy cells. Furthermore, the spectral separation of these two dyes minimizes fluorescence overlap.

Note: The optimal detection settings for MitoView™ 633 are the same as for Cy®5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy®3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes.

See our full selection of NucView™ substrates and kits. For more information about NucView™, see the NucView flyer and reference list under the downloads tab, or see the NucView FAQs.

NucView™ enzyme substrate technology is covered by U.S. Patents.

 

Untreated Jurkat cells stained with the NucView 488 and MitoView 633 Apoptosis Kit. Healthy cells with intact mitochondrial membrane potential stain with far red MitoView 633 mitochondrial membrane potential dye (red), while apoptotic cells stain green with NucView 488 caspase-3 substrate, and do not stain with MitoView 633.
Untreated Jurkat cells stained with the NucView 488 and MitoView 633 Apoptosis Kit. Healthy cells with intact mitochondrial membrane potential stain with far red MitoView 633 mitochondrial membrane potential dye (red), while apoptotic cells stain green with NucView 488 caspase-3 substrate, and do not stain with MitoView 633.

References

1. Cen, et al. DEVD-NucView488: a novel class of enzyme substrates for real-time detection of caspase-3 activity in live cells. The FASEB Journal published online February 8, 2008.

2. Journal of Membrane Science (2014), http://dx.doi.org/10.1016/j.memsci.2014.12.041

3. Journal of Membrane Science (2017), http://dx.doi.org/10.1016/j.memsci.2017.08.036

Download a full list of references: Validated cell lines for NucView 488 with references

 

 

You may also like…

Support & Faq

NucView™ Caspase 3 Enzyme Substrates

The substrate is very stable. Some users have reported performing time course assays with NucView™ 488 Caspase-3 Substrate for 4-5 days.

Category: NucView™ Caspase 3 Enzyme Substrates

← FAQs

NucView™ Caspase-3 Substrates can be added to the cells at the start of the experiment or at the end. A major advantage of NucView™ Caspase-3 Substrates compared to other apoptosis assays is that it can be used to monitor capase-3 activity in real time.

Category: NucView™ Caspase 3 Enzyme Substrates

← FAQs

Yes. NucView™ Caspase-3 Substrates are compatible with flow cytometers and other instruments with the appropriate excitation and emission settings.

Category: NucView™ Caspase 3 Enzyme Substrates

← FAQs

← FAQs

Ask a question

Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.