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NucView® 488 & MitoView™ 633 Apoptosis Assay Kit

No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.

Product Attributes

Apoptosis/viability marker

Caspase, Mitochondrial potential

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy, Live cell imaging, Flow cytometry

Assay type/options

Endpoint assay, Long term staining (24-72h), No-wash staining, Real-time imaging

Substrate specificity

Caspases

Colors

Green/Far-red

Storage Conditions

Store at 2 to 8 °C, Protect from light

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100 assays
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Product Description

This kit contains NucView® 488 Caspase-3 Substrate to detect apoptotic cells and MitoView™ 633 mitochondrial membrane potential dye.

  • Rapid, no-wash, endpoint or real-time assay
  • Non-toxic, allowing multi-day experiments to be performed
  • For flow cytometry, microscopy or live cell imaging systems
  • Dual detection of caspase activity (green) and mitochondrial membrane potential (red/far-red)

NucView® 488 Caspase-3 Substrate is able to detect both intracellular caspase-3 and at the same time stain the cell nucleus, which is known to undergo morphological changes during apoptosis. MitoView™ 633 is a far-red fluorescent mitochondrial dye. The staining is dependent upon the mitochondrial membrane potential; thus, apoptotic cells exhibit a much lower MitoView™ 633 dye staining compared to healthy cells. Furthermore, the spectral separation of these two dyes minimizes fluorescence overlap. See a video of NucView® & MitoView™ in real-time imaging.

Note: The optimal detection settings for MitoView™ 633 are the same as for Cy®5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy®3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes.

To learn about the advantages of monitoring apoptosis using NucView® caspase-3 substrates, visit the NucView® Technology Page.

NucView is a registered trademark of Biotium. NucView enzyme substrate technology is covered by U.S. patents. Cy dye is a registered trademark of GE Healthcare.

References

1. Biochim Biophys Acta (2013) 1833, 2057–2069. http://dx.doi.org/10.1016/j.bbamcr.2013.04.011
2. Front Cell Neurosci (2014) 8, 221. https://doi.org/10.3389/fncel.2014.00221
3. J Cont Release (2014) 183, 94-104. https://doi.org/10.1016/j.jconrel.2014.03.022
4. J Biotech (2015) 213, 97-108. https://doi.org/10.1016/j.jbiotec.2015.02.023
5. J Membrane Sci (2015) 478, 117–129. https://doi.org/10.1016/j.memsci.2014.12.041
6. J Membrane Sci (2017) 542, 399-407. http://dx.doi.org/10.1016/j.memsci.2017.08.036
7. Chemosphere (2018) 212, 307-318. https://doi.org/10.1016/j.chemosphere.2018.08.095
8. Carcinogenesis (2019) pii: bgz137. doi: 10.1093/carcin/bgz137
9. Cancers (2020) 12(3), 529. https://doi.org/10.3390/cancers12030529
10. J Invest Dermatol (2020). https://doi.org/10.1016/j.jid.2020.08.023

Find a list of NucView® references and a list of validated cell lines under Supporting Documents.

 

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