Support & Resources


NucView® 488 & MitoView™ 633 Apoptosis Assay Kit

No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.

Product Attributes

Apoptosis/viability marker

Caspase, Mitochondrial potential

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy, Live cell imaging, Flow cytometry

Assay type/options

Endpoint assay, Long term staining (24-72h), No-wash staining, Real-time imaging

Substrate specificity




Storage Conditions

Store at 2 to 8 °C, Protect from light

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Catalog #
100 assays
Clear selection
Add to wishlist
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

This kit contains NucView® 488 Caspase-3 Substrate to detect apoptotic cells and MitoView™ 633 mitochondrial membrane potential dye.

  • Rapid, no-wash, endpoint or real-time assay
  • Non-toxic, allowing multi-day experiments to be performed
  • For flow cytometry, microscopy or live cell imaging systems
  • Dual detection of caspase activity (green) and mitochondrial membrane potential (red/far-red)

NucView® 488 Caspase-3 Substrate is able to detect both intracellular caspase-3 and at the same time stain the cell nucleus, which is known to undergo morphological changes during apoptosis. MitoView™ 633 is a far-red fluorescent mitochondrial dye. The staining is dependent upon the mitochondrial membrane potential; thus, apoptotic cells exhibit a much lower MitoView™ 633 dye staining compared to healthy cells. Furthermore, the spectral separation of these two dyes minimizes fluorescence overlap. See a video of NucView® & MitoView™ in real-time imaging.

Note: The optimal detection settings for MitoView™ 633 are the same as for Cy®5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy®3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes.

To learn about the advantages of monitoring apoptosis using NucView® caspase-3 substrates, visit the NucView® Technology Page.

NucView is a registered trademark of Biotium. NucView enzyme substrate technology is covered by U.S. patents. Cy dye is a registered trademark of GE Healthcare.


1. Biochim Biophys Acta (2013) 1833, 2057–2069.
2. Front Cell Neurosci (2014) 8, 221.
3. J Cont Release (2014) 183, 94-104.
4. J Biotech (2015) 213, 97-108.
5. J Membrane Sci (2015) 478, 117–129.
6. J Membrane Sci (2017) 542, 399-407.
7. Chemosphere (2018) 212, 307-318.
8. Carcinogenesis (2019) pii: bgz137. doi: 10.1093/carcin/bgz137
9. Cancers (2020) 12(3), 529.
10. J Invest Dermatol (2020).

Find a list of NucView® references and a list of validated cell lines under Supporting Documents.


You may also like…