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NucView® 488 and RedDot™ 2 Apoptosis and Necrosis Kit

NucView®488 and RedDot™2 Apoptosis/Necrosis Assay Kit contains NucView®488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity.

Product Attributes

Apoptosis/viability marker

Caspase, Dead cell stain, Apoptosis/necrosis assay

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy, Live cell imaging, Flow cytometry

Assay type/options

Endpoint assay, No-wash staining

Substrate specificity

Caspases

Colors

Green/Far-red

Size
Catalog #
price
Qty
100 assays
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Product Description

NucView®488 and RedDot™ Apoptosis/Necrosis Assay Kit contains NucView®488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™ is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView®488 Caspase-3 Substrate can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright green fluorescence. Thus, NucView®488 Caspase-3 Substrate allows detection caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis.

RedDot™ is a cell membrane-impermeable, far-red dye with high selectivity for membrane compromised or dead cells. RedDot™ has far-red emission at 695 nm for detection in the Cy® channel, well-separated from the green fluorescence of NucView®488. The excitation maximum of RedDot™ is 665 nm, but it can be efficiently excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line.

Note: while NucView®488 staining is formaldehyde-fixable and compatible with subsequent immunostaining, fixation after staining with RedDot™ is not recommended because it can result in increased background staining of healthy cells.

See our full selection of NucView® substrates and kits.

NucView® enzyme substrate technology is covered by U.S. patents.
Cy5 is a registered trademark of GE Healthcare.

References

 

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