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What is the difference between the NucView® 488 Kit and the NucView® 488 Substrates?

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The NucView® 488 Assay Kit for Live Cells (30029) contains NucView® 488 substrate at 0.2 mM in DMSO and caspase-3 inhibitor Ac-DEVD-CHO. The final DMSO concentration in the kit assay can be fairly high, which is undesirable for sensitive cell types. Because of this, we offer NucView® 488 Caspase-3 Substrate at 1 mM in DMSO (10402) and at 1 mM in PBS (10403), so customers can control the amount of DMSO in their assay. Note that in non-DMSO sensitive cell types, adding DMSO during the substrate incubation can enhance NucView® staining.

We also offer blue fluorogenic NucView® 405 in DMSO (10405) and PBS (10407), and orange fluorogenic NucView® 530 in DMSO (10406) and PBS (10408).

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