Permeabilization and Blocking Buffer (5X)
A concentrated 5X buffer solution for one step permeabilization and blocking for immunofluorescence staining for fluorescence microscopy.
This product is available by special order only.
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Product Description
The Permeabilization and Blocking Buffer (5X) is a concentrated stock solution for one step cell permeabilization and blocking. It is designed for use during immunofluorescence staining for fluorescence microscopy.
- Permeabilizes fixed cells for immunofluorescence staining
- Blocks non-specific binding of antibodies
- Can be used to dilute antibodies for staining, and as a wash buffer
- Contains goat serum, not for use with anti-goat or anti-sheep antibodies
- 100 mL of 5X solution
Note: this buffer contains goat serum and therefore is not compatible with anti-goat and anti-sheep secondary antibodies. We recommend Permeabilization Buffer (22016) and 10% Fish Gelatin Blocking Agent (22010) for use with anti-goat and anti-sheep secondary antibodies.
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