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Choose Your Mounting Media
| Product | Nuclear Counterstain | Cat. No. | Features |
|---|---|---|---|
| EverBrite™ Mounting Medium | None | 23001 | • Wet-set mounting medium • Requires coverslip sealing • Refractive index 1.46 |
| EverBrite™ with DAPI | DAPI | 23002 | |
| Drop-n-Stain EverBrite™ Mounting Medium | None | 23008 | • Wet-set mounting medium • Convenient dropper bottle • Ideal for wells & chambers • Refractive index 1.42 |
| Drop-n-Stain EverBrite™ with DAPI | DAPI | 23009 | |
| EverBrite™ Hardset Mounting Medium | None | 23003 | • Hard-set mounting medium • Forms hard seal after 24 h • No coverslip sealing needed • Refractive index 1.42 after 24 h of curing, and 1.46 four days after curing |
| EverBrite™ Hardset with DAPI | DAPI | 23004 | |
| EverBrite™ Hardset with NucSpot® 640 | NucSpot® 640 | 23016 | |
| EverBrite TrueBlack® Hardset Mounting Medium | None | 23017 | • Unique antifade with lipofuscin quenching • Quenches as it hardens, with low background • Refractive index 1.42 after 24 h of curing, and 1.46 four days after curing |
| EverBrite TrueBlack® Hardset with DAPI | DAPI | 23018 | |
| EverBrite TrueBlack® Hardset with NucSpot® 640 | NucSpot® 640 | 23019 | |
| CoverGrip™ Coverslip Sealant | N/A | 23005 | • For sealing edges of wet-set coverslips |
Original EverBrite™ Mounting Medium
Biotium’s EverBrite™ line of mounting media are formulated to preserve fluorescence of our CF® Dyes and other fluorochromes. EverBrite™ comes in wet-set and hardset formulations, with and without nuclear counterstains. Drop-n-Stain EverBrite™ provides the wet-set EverBrite™ in a dropper bottle for easy dispensing.
Features
- Antifade mounting medium optimally formulated to prevent photobleaching of fluorophores
- Refractive index is well-matched to that of coverslip glass and immersion oil
- Available in wet-set or hardset
- Wet-set formulation available as convenient Drop-n-Stain EverBrite™
- Available with DAPI, hardest formulation available with NucSpot® 640
EverBrite™ Hardset Mounting Medium with NucSpot®
Clearer Counterstaining in the Far-Red Channel
EverBrite™ Hardset with far-red NucSpot® 640 counterstain is an antifade medium that allows for one-step mounting and nuclear counterstaining. NucSpot® 640 is a far-red DNA binding dye for the Cy®5 channel that offers greater nuclear-specificity than the classic dye propidium iodide (PI) and avoids problems with cross-talk and photoconversion from DAPI.
Features
- One-step mounting and nuclear counterstaining
- Avoid issues of cross-talk and photoconversion from DAPI
- Superior nuclear specificity compared to PI
- Also available with EverBrite TrueBlack® Hardset
EverBrite TrueBlack® Hardset Mounting Medium
The all-in-one mounting medium
Lipofuscin are autofluorescent granules that are a common source of background that make fluorescence imaging virtually impossible in human and aged animal tissues such as brain and retina. EverBrite TrueBlack® Hardset Mounting Medium is the only mounting medium optimally formulated for quenching lipofuscin fluorescence while offering the same protection against photobleaching as our original EverBrite™ Hardset. The mounting medium is available without nuclear stain, with DAPI, or with NucSpot® 640 nuclear counterstains.
Features
- The only mounting medium with autofluorescence quenching
- Quenches as it hardens, with low background fluorescence
- Optimally formulated for protecting CF® Dyes and other dyes from photobleaching
- Refractive index well-matched to coverglass (1.46 after curing)
- Choice of DAPI, NucSpot® 640, or without nuclear counterstain
