Labeled Nucleotides

DEAC-dUTP (diethylaminocoumarin-dUTP) can be used to synthesize fluorescent DNA probes for in situ hybridization, microarray or blotting techniques. DEAC has absorption at 426 nm and emission at 480 nm.

Fluorescein-12-dUTP can be used to synthesize fluorescent DNA probes.

BrdU (5-Bromo-2′-deoxyuridine) can be incorporated into DNA during cell division and subsequently detected by a BrdU antibody. The probe can be used to study cell-cycle kinetics (1).

5-Bromo-dUTP (5-Bromo-2′-deoxyuridine-5′-triphosphate) is widely used in TUNEL assay to detect apoptosis cells, and also is a good substrate for reverse transcriptase.

BrUTP (5-Bromouridine-5′-triphosphate) can be enzymatically incorporated into RNA and detected with anti-BrdU antibodies.

Biotin-11-dCTP (Biotin-11-2′-deoxycytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number 11 is the number of atoms in the linker between biotin and dCTP.

This product is similar to biotin-11-UTP (40033) and biotin-16-UTP (40023) except that it has a longer and water soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.

Biotin-11-CTP (Biotin-11-cytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into RNA.

Biotin-11-UTP can be enzymatically incorporated into RNA. The number 11 is the number of atoms in the linker between biotin and UTP.

This product is similar to biotin-11-dUTP (40029) and biotin-16-dUTP (40022) except that it has a longer and water-soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.

This product is similar to biotin-11-dUTP (40029) and biotin-16-dUTP (40022) except that it has a longer and water-soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.

Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number ’11’ is the number of atoms in the linker between biotin and dUTP.

Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number ’11’ is the number of atoms in the linker between biotin and dUTP.

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′ end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies.

Biotin-16-UTP can be enzymatically incorporated into RNA. The number 16 indicates the number of atoms between biotin and UTP.

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′ end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies.