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CFDA SE, 5 and 6-isomers

5(6)-CFDA, SE (full name: 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester, also known as CFSE) is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and non-fluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

Product Attributes

pKa

pKa 6.5

CAS number

150347-59-4

Applications

pH indicators

Cell permeability

Membrane permeant

Cellular localization

Nucleus & cytoplasm

Colors

Green

Excitation/Emission

495/519 nm (pH 7) (end product)

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25 mg
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Product Description

5(6)-CFDA, SE (full name: 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester, also known as CFSE) is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and non-fluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases. The labeling has been shown to be stable to formaldehyde or gluteraldehyde fixation. Also see our CFDA SE Cell Proliferation Assay Kit (catalog no. 30050).

  • λExEm (of hydrolysis product at pH 7) = 495/519 nm
  • White solid soluble in DMSO or DMF
  • Store at -20°C and protect from light
  • C29H19NO11
  • MW: 557
  • [150347-59-4]

References

1. J Cell Biol 101, 610 (1985).

2. J Cell Biol 103, 2649 (1986).

3. Cell Transplantation 3, 397 (1994).

 

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