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Apoptotic, Necrotic and Healthy Cells Quantitation Kit Plus

A convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.

Product Attributes

Apoptosis/viability marker

Phosphatidylserine/Annexin V, Dead cell stain, All cell stain, Apoptosis/necrosis assay

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy

Assay type/options

Endpoint assay



Catalog #
50 assays
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Product Description

The Apoptotic, Necrotic & Healthy Cells Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.

Apoptosis and necrosis are two major processes by which cells die. Apoptosis is an active, genetically regulated disassembly of the cell from within. During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane for phagocytic cell recognition. The human anticoagulant Annexin V is a 35 kDa, Ca2 -dependent phospholipid-binding protein with high affinity for PS. Annexin V labeled with CF®488A stains apoptotic cells green by binding to PS exposed on the cell surface. CF®488A is spectrally similar to fluorescein (FITC), with much brighter and more photostable fluorescence.

Necrosis normally results from severe cellular insult. Both internal organelle and plasma membrane integrity are lost, resulting in the release of cellular contents into the surrounding environment. Ethidium homodimer III (EthD-III) is a highly positively charged nucleic acid probe, which is impermeant to live cells, but stains cells with compromised membrane integrity with red fluorescence. EthD-III is a superior alternative to propidium iodide (PI) or ethidium homodimer I because of its significantly higher affinity for DNA and higher fluorescence quantum yield.

The kit also includes the membrane permeable DNA dye Hoechst 33342 to stain all cell nuclei blue.


  • Simple: Simultaneous detection of apoptotic, necrotic, and total cells in a single assay
  • Fast: A single 15-30 min staining step
  • Sensitive: CF®488A Annexin V is brighter and more photostable than FITC Annexin V, while EthD-III is a superior alternative to both PI and EthD-I for staining necrotic cells.

Kit Components:

  • CF®488A-Annexin V
  • Ethidium homodimer III (EthD-III)
  • Hoechst 33342
  • 5X binding buffer

Spectral properties (Ex/Em):

  • CF®488A Annexin V: 490/515 nm
  • Ethidium homodimer III (EthD-III) (with DNA): 522/593 nm (EthD-III also has a large absorption peak at 279 nm)
  • Hoechst: 350/461 nm


1. Cvjeticanin, T., et al. T Cells Cooperate with Palmitic Acid in Induction of Beta Cell Apoptosis. BMC Immunology doi:10.1186/1471-2172-10-29 (2010).


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