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CF®488A Annexin V and 7-AAD Apoptosis Kit

This kit contains CF®488A Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells for flow cytometry.

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100 assays
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Product Description

This kit contains CF®488A Annexin V for staining apoptotic cells green, and 7-AAD for staining necrotic cells with far-red fluorescence, for detection by flow cytometry.

Features

  • Simultaneous detection of apoptotic & necrotic cells
  • 15-30 minute staining
  • Next-generation CF®488A is brighter and more photostable than FITC
  • For flow cytometry

Kit Components

  • CF®488A Annexin V (human;recombinant, produced in E. coli)
  • 7-AAD
  • 5X Annexin Binding Buffer

Spectral Properties

  • CF®488A Annexin V: Ex/Em 490/515 nm(FITC channel)
  • 7-AAD: Ex/Em 548/648 nm (with DNA)(PE-Cy® or PerCP channel)

In apoptotic cells, the phospholipid phosphatidylserine (PS) is translocated from the inner to the outer surface of the plasma membrane, which targets the dying cells for phagocytosis. The human anticoagulant, Annexin V, is a 35 kDa Ca2 -dependent phospholipid binding protein with a high affinity for PS. Annexin V labeled with CF®488A labels apoptotic cells with green fluorescence by binding to PS exposed on the outer leaflet. Our CF®488A dye is superior to fluorescein/FITC because it is not affected by pH and has far better photostability.

7-AAD is a membrane-impermeant nucleic acid dye that is excluded by live cells, but stains necrotic cells and late apoptotic cells. 7-AAD (Abs/Em with DNA = 543/655 nm) emits in the far-red spectrum and can be well separated from CF®488A and other green emitting dyes using the 488 nm line of a flow cytometer.

See our full selection of Cell Viability & Apoptosis Assays.

CF dye technology is covered by US and international patents. Cy Dye is a registered trademark of Cytiva.

Product Attributes

Apoptosis/viability marker
Phosphatidylserine/Annexin V, Dead cell stain, Apoptosis/necrosis assay
For live or fixed cells
For live/intact cells
Detection method/readout
Flow cytometry
Assay type/options
Endpoint assay
Size
100 assays
Colors
Green/Far-red
Product origin
Annexin V (human); recombinant, produced in E. coli
Storage Conditions
Store at 2 to 8 °C, Do not freeze, Protect from light

Documents, Protocols, SDS and COA

References

Download a list of curated CF® Dye references.

Citations

Download a list of curated CF® Dye references.

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FAQs

Apoptosis, Necrosis, and Cell Viability Kits

It has been reported in publications that concentrations of serum above 10% in the assay may affect the results.

See the following publications for more information

https://doi.org/10.2144/04373ST05

https://dx.doi.org/10.1007%2Fs10616-007-9057-4

Our ViaFluor® SE Cell Proliferation assay is a dye dilution assay for cell division, like CFSE and CellTrace™ Violet from Thermo. This type of assay is commonly used to measure lymphocyte proliferative responses in culture and in vivo (if the labeled cells are injected back into mice). It requires flow cytometry to analyze and allows you to count how many cell divisions have occurred in the labeled cells.

For more information and a typical procedure for using fluorescent ViaFluor® SE Dyes with PMBCs, which can easily be adapted for use with other cell types, please see our Tech Tip: Measuring Cell Division in PMBCs by Flow Cytometry

If flow cytometry is not an option, we offer absorbance-based and fluorescence-based microplate assays for quantitating cell numbers. These measure mitochondrial activity (resazurin/MTT/XTT) or intracellular esterase activity (calcein AM) as a readout of viable cell numbers. Please visit the Cell Viability and Apoptosis technology page for more information.

The ATP-Glo™ assay is a luminescence assay for cellular ATP levels, which are proportional to the number of live cells. This assay requires a luminometer.

CellTrace is a trademark of Thermo Fisher Scientific.

Our Resazurin Cell Viability Assay (Cat. No. 30025) has red fluorescence (Ex/Em 530-560/590 nm), and is specifically designed for microplate reader. It is an economical, easy-to-use, and homogeneous (no-wash) assay for quantifying live cells. It is similar to alamarBlue®, PrestoBlue®, and CellTiter-Blue®.

The Calcein AM Cell Viability Assay (Cat. No. 30026) has green fluorescence (Ex/Em 485/530 nm), and also works well for microplate reader. This assay requires culture medium to be removed from cells before adding the viability dye in buffer. We also offer the Viability/Cytotoxicity Assay for Animal Live & Dead Cells, which combines calcein-AM with the fluorescent dead cell stain EthD-III, and is compatible with microplate reader.

alamarBlue is a registered trademark of Trek Biosystems. CellTiter-Blue is a registered trademark of Promega Corporation. PrestoBlue is a registered trademark of Thermo Fisher Scientific.
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