Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

Apoptotic, Necrotic, and Healthy Cells Quantification Kit

A convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.

Product Attributes

Apoptosis/viability marker

Phosphatidylserine/Annexin V, Dead cell stain, All cell stain, Apoptosis/necrosis assay

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy

Assay type/options

Endpoint assay

Colors

Blue/Green/Red

Size
Catalog #
price
Qty
50 assays
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

The Apoptotic, Necrotic & Healthy Cells Quantification Kit provides a convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.

Apoptosis and necrosis are two major processes by which cells die. Apoptosis is an active, genetically regulated disassembly of the cell from within. During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane for phagocytic cell recognition. The human anticoagulant Annexin V is a 35 kDa, Ca2 -dependent phospholipid-binding protein with high affinity for PS. Annexin V labeled with fluorescein (FITC) stains apoptotic cells green by binding to PS exposed on the cell surface.

Necrosis normally results from severe cellular insult. Both internal organelle and plasma membrane integrity are lost, resulting in the release of cellular contents into the surrounding environment. Ethidium homodimer III (EthD-III) is a highly positively charged nucleic acid probe, which is impermeant to live cells, but stains cells with compromised membrane integrity with red fluorescence. EthD-III is a superior alternative to propidium iodide (PI) or ethidium homodimer I because of its significantly higher affinity for DNA and higher fluorescence quantum yield.

The kit also includes the membrane permeable DNA dye Hoechst 33342 to stain all cell nuclei blue.

Features:

  • Simple: Simultaneous detection of apoptotic, necrotic and healthy cells in a single assay
  • Fast: A single 15-30 min staining step
  • Sensitive: EthD-III is a superior alternative to both PI and EthD-I for staining necrotic cells.

Kit Components:

  • FITC Annexin V
  • Ethidium Homodimer III
  • Hoechst 33342
  • 5X binding Buffer

Spectral properties (Ex/Em):

  • FITC Annexin V: 498/517 nm
  • Ethidium homodimer III (EthD-III) (with DNA): 522/593 nm (EthD-III also has a large absorption peak at 279 nm)
  • Hoechst: 350/461 nm

Also see the Apoptosis and Necrosis Quantitation Kit Plus (catalog no. 30065) and Apoptotic, Necrotic, and Healthy Cells Quantitation Kit Plus (catalog no. 30066), which feature Annexin V conjugated to Biotium’s superior green fluorescent dye CF®488A, which is brighter and more photostable than FITC.

References

1. Physical Biology (2011) doi:10.1088/1478-3975/8/1/015006

2. Pharmacological Reports 63, 139 (2011).

3. International Journal of Molecular Sciences 9, 56 (2008)

.

4. Science 300, 91 (2003).

5. J Neurosci Methods. 36, 229 (1991).

6. FEBS Lett 203, 99 (1986).

7. Biosci. Rep. 7, 289 (1987).

 

You may also like…

    NucView® 488 Caspase-3 Enzyme Substrate, 1 mM in PBS

    NucView® 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time.

    NucView® 405 Caspase-3 Substrate, 1 mM in DMSO

    NucView® 405 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

    NucView® 530 Caspase-3 Substrate, 1 mM in DMSO

    NucView® 530 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

    Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate & Annexin V

    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugated with your choice of red or far-red dye. The kit is used for studying two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation.

    Staurosporine

    Staurosporine is a potent and cell-permeable inhibitor of a wide variety of protein kinases (1,2) including protein kinase C (IC50 = 0.7 nM), protein kinase A (IC50 = 7 nM), and protein kinase G (IC50 = 8.5 nM). Staurosporine induces apoptosis in human neuroblastoma cells and chick embryonic neurons (3,4).

    Live-or-Dye™ Fixable Viability Staining Kits

    Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.