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Apoptotic, Necrotic, and Healthy Cells Quantification Kit

A convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by fluorescence microscopy.

Product Attributes

Apoptosis/viability marker

Phosphatidylserine/Annexin V, Dead cell stain, All cell stain, Apoptosis/necrosis assay

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy

Assay type/options

Endpoint assay

Colors

Blue/Green/Red

Product origin

Annexin V (human); recombinant, produced in E. coli

Storage Conditions

Store at 2 to 8 °C, Do not freeze, Protect from light

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price
Qty
50 assays
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Product Description

The Apoptotic, Necrotic & Healthy Cells Quantification Kit provides a convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by fluorescence microscopy.

Features

  • Simultaneous detection of apoptotic, necrotic, and all cells
  • 15-30 minute staining
  • EthD-III is a superior alternative to PI and EthD-I

Spectral Properties

  • FITC Annexin V: Ex/Em 498/517 nm
  • EthD-III: Ex/Em 279, 522/593 nm (with DNA)
  • Hoechst 33342: Ex/Em 352/461 nm (with DNA)

Kit Components

  • FITC Annexin V (human; recombinant, produced in E. coli)
  • Ethidium Homodimer III
  • Hoechst 33342
  • 5X Annexin Binding Buffer

Apoptosis and necrosis are two major processes by which cells die. Apoptosis is an active, genetically regulated disassembly of the cell from within. During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane for phagocytic cell recognition. The human anticoagulant Annexin V is a 35 kDa, Ca2+-dependent phospholipid-binding protein with high affinity for PS. Annexin V labeled with fluorescein (FITC) stains apoptotic cells green by binding to PS exposed on the cell surface.

Necrosis normally results from severe cellular insult. Both internal organelle and plasma membrane integrity are lost, resulting in the release of cellular contents into the surrounding environment. Ethidium homodimer III (EthD-III) is a highly positively charged nucleic acid probe, which is impermeant to live cells, but stains cells with compromised membrane integrity with red fluorescence. EthD-III is a superior alternative to propidium iodide (PI) or ethidium homodimer I because of its significantly higher affinity for DNA and higher fluorescence quantum yield.

The kit also includes the cell membrane-permeant DNA dye Hoechst 33342 to stain all cell nuclei blue.

Also see the Apoptosis and Necrosis Quantification Kit Plus and Apoptotic, Necrotic, and Healthy Cells Quantification Kit Plus, which feature Annexin V conjugated to Biotium’s superior green fluorescent dye CF®488A, which is brighter and more photostable than FITC.

See our full selection of Cell Viability & Apoptosis Assays and Viability & Apoptosis Assays for Flow Cytometry.

References

1. Int. J. Mol. Sci. (2008) 9(1), 56-64. https://doi.org/10.3390/ijms9010056
2. Physical Biology (2011) 8, 015006. doi:10.1088/1478-3975/8/1/015006
3. Pharmacological Reports (2011) 63, 139. https://doi.org/10.1016/S1734-1140(11)70408-X
4. Photodiag Photodynamic Ther (2015)12(1), 27-35. https://doi.org/10.1016/j.pdpdt.2014.12.013
5. Photodiag Photodynamic Ther (2018) 23, 240–243. https://doi.org/10.1016/j.pdpdt.2018.07.007

 

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