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CF®488A Annexin V and PI Apoptosis Kit

This kit contains CF®488A Annexin V to stain apoptotic cells green and propidium iodide (PI) to stain necrotic cells red for fluorescence microscopy or flow cytometry.

Product Attributes

Apoptosis/viability marker

Phosphatidylserine/Annexin V, Dead cell stain, Apoptosis/necrosis assay

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay

Colors

Green/Red

Product origin

Annexin V (human); recombinant, produced in E. coli

Storage Conditions

Store at 2 to 8 °C, Do not freeze, Protect from light

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Technology

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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100 assays
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Product Description

This kit contains CF®488A Annexin V for staining apoptotic cells green, and propidium iodide (PI) for staining necrotic cells with red fluorescence, for detection by flow cytometry or fluorescence microscopy.

Features

  • Simultaneous detection of apoptotic & necrotic cells
  • 15-30 minute staining
  • Next-generation CF®488A is brighter and more photostable than FITC
  • For fluorescence microscopy or flow cytometry

Kit Components

  • CF®488A Annexin V (human;recombinant, produced in E. coli)
  • Propidium Iodide
  • 5X Annexin Binding Buffer

Spectral Properties

  • CF®488A Annexin V: Ex/Em 490/515 nm
  • PI: Ex/Em 530/622 nm (with DNA)

In apoptotic cells, the phospholipid phosphatidylserine (PS) is translocated from the inner to the outer surface of the plasma membrane, which targets the dying cells for phagocytosis. The human anticoagulant, Annexin V, is a 35 kDa Ca2 -dependent phospholipid binding protein with a high affinity for PS. Annexin V labeled with CF®488A labels apoptotic cells with green fluorescence by binding to PS exposed on the outer leaflet. Our CF®488A dye is superior to fluorescein/FITC because it is not affected by pH and has far better photostability.

Propidium iodide (PI) is impermeant to live cells or early apoptotic cells, but stains necrotic cells and late apoptotic cells with red fluorescence. Both PI and CF®488A Annexin V can be excited using the 488 nm flow cytometry laser line.

See our full selection of Cell Viability & Apoptosis Assays.

 

CF dye technology is covered by US and international patents.

References

1. Saudi J Biol Sci (2013) 20(2), 131-139. https://doi.org/10.1016/j.sjbs.2012.12.002
2. Cell Death Disease (2014) 5, e1027. doi:10.1038/cddis.2013.550
3. Med Chem Com (2014) 5, 1404-1409. DOI: 10.1039/c4md00161c
4. PLoS ONE (2014) 9(12), e115652. doi:10.1371/journal.pone.0115652
5. RSC Adv (2014) 4, 7062. DOI: 10.1039/c3ra45333b
6. Cell Biochem Biophys (2015) 71, 1525–1536. DOI 10.1007/s12013-014-0376-8
7. New J Chem (2015) 39, 224. DOI: 10.1039/c4nj00936c
8. Autophagy (2016) 12(4), 632-647. DOI:10.1080/15548627.2016.1147667
9. Adv Sci (2017) 5(3), 1700481. doi: 10.1002/advs.201700481
10. Mol Med Rep (2017) 15, 3566-3574. DOI: 10.3892/mmr.2017.6480
11. Biol Pharm Bull (2018) 41, 458–464. doi: 10.1248/bpb.b17-00449
12. J Appl Toxicol (2018) 38(3), 366–375. doi:10.1002/jat.3546
13. J Cell Sci (2018) 131, jcs216671. doi: 10.1242/jcs.216671
14. Mol Cancer (2018) 17, 72. https://doi.org/10.1186/s12943-018-0821-1

 

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