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CF®488A Annexin V and PI Apoptosis Kit

This kit contains CF488A-Annexin V to stain apoptotic cells and PI to stain necrotic cells.

Product Attributes

Apoptosis/viability marker

Phosphatidylserine/Annexin V, Dead cell stain, Apoptosis/necrosis assay

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay

Colors

Green/Red

Size
Catalog #
price
Qty
100 assays
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Product Description

Phosphatidylserine (PS) is translocated from the inner to the outer surface of the cell for phagocytic cell recognition. The human anticoagulant, Annexin V, is a 35 kDa Ca2 -dependent phospholipid protein with a high affinity for PS. Annexin V labeled with CF®488A (Abs/Em= 490/515 nm) can identify apoptotic cells in green by binding to PS exposed on the outer leaflet. Our CF®488A dye is superior to fluorescein/FITC as it is not affected by pH and has much better photostability. Propidium iodide (PI) is impermeant to live cells or early apoptotic cells, but stains necrotic cells and late apoptotic cells with red fluorescence (Abs/Em with DNA = 535/617 nm). Both PI and Annexin V-CF®488A can be excited using the 488nm line of a flow cytometer.

References

1. Cell Death and Disease (2014), doi:10.1038/cddis.2013.550

2. Autophagy (2016), doi: 10.1080/15548627.2016.1147667

3. J Young Pharm. (2016), doi: 10.5530/jyp.2016.3.4

4. Molecular Medicine Reports (2017), doi: 10.3892/mmr.2017.6480

 

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