CF®488A Annexin V and PI Apoptosis Kit
This kit contains CF®488A Annexin V to stain apoptotic cells green and propidium iodide (PI) to stain necrotic cells red for fluorescence microscopy or flow cytometry.
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This kit contains CF®488A Annexin V for staining apoptotic cells green, and propidium iodide (PI) for staining necrotic cells with red fluorescence, for detection by flow cytometry or fluorescence microscopy.
Features
- Simultaneous detection of apoptotic & necrotic cells
- 15-30 minute staining
- Next-generation CF®488A is brighter and more photostable than FITC
- For fluorescence microscopy or flow cytometry
Kit Components
- CF®488A Annexin V (human;recombinant, produced in E. coli)
- Propidium Iodide
- 5X Annexin Binding Buffer
Spectral Properties
- CF®488A Annexin V: Ex/Em 490/515 nm
- PI: Ex/Em 530/622 nm (with DNA)
In apoptotic cells, the phospholipid phosphatidylserine (PS) is translocated from the inner to the outer surface of the plasma membrane, which targets the dying cells for phagocytosis. The human anticoagulant, Annexin V, is a 35 kDa Ca2 -dependent phospholipid binding protein with a high affinity for PS. Annexin V labeled with CF®488A labels apoptotic cells with green fluorescence by binding to PS exposed on the outer leaflet. Our CF®488A dye is superior to fluorescein/FITC because it is not affected by pH and has far better photostability.
Propidium iodide (PI) is impermeant to live cells or early apoptotic cells, but stains necrotic cells and late apoptotic cells with red fluorescence. Both PI and CF®488A Annexin V can be excited using the 488 nm flow cytometry laser line.
See our full selection of Cell Viability & Apoptosis Assays.
References
Download a list of curated CF® Dye references.