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CF®488A Annexin V and 7-AAD Apoptosis Kit

This kit contains CF488A-Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells.

Product Attributes

Apoptosis/viability marker

Phosphatidylserine/Annexin V, Dead cell stain, Apoptosis/necrosis assay

For live or fixed cells

For live/intact cells

Detection method/readout

Flow cytometry

Assay type/options

Endpoint assay

Colors

Green/Far-red

Size
Catalog #
price
Qty
100 assays
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Product Description

This kit contains CF®488A Annexin V for staining apoptotic cells green, and 7-AAD for staining necrotic cells with far-red fluorescence, for detection by flow cytometry or fluorescence microscopy.

Phosphatidylserine (PS) is translocated from the inner to the outer surface of apoptotic cells for phagocytic cell recognition. The human anticoagulant, Annexin V, is a 35 kDa Ca2 -dependent phospholipid protein with a high affinity for PS. Annexin V labeled with CF®488A (Abs/Em= 490/515 nm) can identify apoptotic cells in green by binding to PS exposed on the outer leaflet. Our CF®488A dye is superior to fluorescein/FITC as it is not affected by pH and has much better photostability.

7-AAD is a membrane-impermeant nucleic acid dye that is excluded by live cells, but stains necrotic cells and late apoptotic cells. 7-AAD (Abs/Em with DNA = 543/655 nm) emits in the far-red spectrum and can be well separated from CF®488A and other green emitting dyes using the 488 nm line of a flow cytometer.

References

 

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