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CF®488A Annexin V and 7-AAD Apoptosis Kit

This kit contains CF®488A Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells for flow cytometry.

Product Attributes

Apoptosis/viability marker

Phosphatidylserine/Annexin V, Dead cell stain, Apoptosis/necrosis assay

For live or fixed cells

For live/intact cells

Detection method/readout

Flow cytometry

Assay type/options

Endpoint assay

Colors

Green/Far-red

Product origin

Annexin V (human); recombinant, produced in E. coli

Storage Conditions

Store at 2 to 8 °C, Do not freeze, Protect from light

Size
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price
Qty
100 assays
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Product Description

This kit contains CF®488A Annexin V for staining apoptotic cells green, and 7-AAD for staining necrotic cells with far-red fluorescence, for detection by flow cytometry.

Features

  • Simultaneous detection of apoptotic & necrotic cells
  • 15-30 minute staining
  • Next-generation CF®488A is brighter and more photostable than FITC
  • For flow cytometry

Kit Components

  • CF®488A Annexin V (human;recombinant, produced in E. coli)
  • 7-AAD
  • 5X Annexin Binding Buffer

Spectral Properties

  • CF®488A Annexin V: Ex/Em 490/515 nm(FITC channel)
  • 7-AAD: Ex/Em 543/655 nm (with DNA)(PE-Cy® or PerCP channel)

In apoptotic cells, the phospholipid phosphatidylserine (PS) is translocated from the inner to the outer surface of the plasma membrane, which targets the dying cells for phagocytosis. The human anticoagulant, Annexin V, is a 35 kDa Ca2 -dependent phospholipid binding protein with a high affinity for PS. Annexin V labeled with CF®488A labels apoptotic cells with green fluorescence by binding to PS exposed on the outer leaflet. Our CF®488A dye is superior to fluorescein/FITC because it is not affected by pH and has far better photostability.

7-AAD is a membrane-impermeant nucleic acid dye that is excluded by live cells, but stains necrotic cells and late apoptotic cells. 7-AAD (Abs/Em with DNA = 543/655 nm) emits in the far-red spectrum and can be well separated from CF®488A and other green emitting dyes using the 488 nm line of a flow cytometer.

See our full selection of Cell Viability & Apoptosis Assays.

CF dye technology is covered by US and international patents. Cy dye is a registered trademark of GE Healthcare.

References

1. PLoS ONE (2016) 11(5), e0155450. doi:10.1371/journal.pone.0155450
2. Int J Nanomed (2018) 13, 6089–6097. doi: 10.2147/IJN.S179095

 

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