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CF®555-ddCTP

An orange-red fluorescent 3′-end chain terminator in Sanger sequencing.

Product Attributes

Excitation/Emission

555/565 nm

Colors

Red

Molecular weight

1383

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25 nmol
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Protocols
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Product Description

ddCTP lacks the 3′ hydroxyl group preventing DNA polymerase from extending the DNA strand. CF®555-ddCTP is an orange-red fluorescent dye conjugate that can be used as 3′-end chain terminator in Sanger sequencing.

  • Orange-red fluorescent dye conjugate
  • Superior brightness and photostability without being excessively charged
  • Much brighter and more photostable than Cy®3, comparable to Alexa Fluor® 555
  • Supplied in lyophilized form

The CF®555 dye conjugated to ddCTP is spectrally similar to Cy®3 and Alexa Fluor® 555. Like Alexa Fluor® 555, CF®555 is much brighter and more photostable than Cy®3. However, unlike Alexa Fluor® 555, which carries 4 negative charges, CF®555 achieves its superior performance without being excessively charged.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

CF is a registered trademark of Biotium, Inc. Cy Dye is a registered trademark of Cytiva; Alexa Fluor is a registered trademark of Thermo Fisher Scientific.

References

Download a list of CF® dye references.

 

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