CF™555 is an orange-red fluorescent dye spectrally similar to Cy®3 and Alexa Fluor®555. Like Alexa Fluor® 555, CF™555 is much brighter and more photostable than Cy®3. However, unlike Alexa Fluor® 555, which carries 4 negative charges, CF™555 achieves its superior performance without being excessively charged. Labeling antibodies with a highly charged dye can dramatically change the isoelectric point of the antibody, leading to increased background staining.
A full selection of reactive dyes, secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, Annexin V and α-bungarotoxin are available for CF™ dyes.
- Abs/Em Maxima: 555/565 nm
- Extinction coefficient: 150,000
- Molecular weight: ~901
- Excitation laser line: 532 nm, 555 nm, or 568 nm
- Direct replacement for: Alexa Fluor® 555, ATTO 550, Cy®3, DyLight® 549, Rhodamine, TRITC