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CF™555 Dye


CF™555 is an orange-red fluorescent dye spectrally similar to Cy®3 and Alexa Fluor®555. Like Alexa Fluor® 555, CF™555 is much brighter and more photostable than Cy®3. However, unlike Alexa Fluor® 555, which carries 4 negative charges, CF™555 achieves its superior performance without being excessively charged. Labeling antibodies with a highly charged dye can dramatically change the isoelectric point of the antibody, leading to increased background staining.

A full selection of reactive dyes, secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, Annexin V and α-bungarotoxin are available for CF™ dyes.

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CF™555 conjugates, labeling kits, and reactive dyes

Technical Summary

  • Abs/Em Maxima: 555/565 nm
  • Extinction coefficient: 150,000
  • Molecular weight: ~901
  • Excitation laser line: 532 nm, 555 nm, or 568 nm
  • Direct replacement for: Alexa Fluor® 555, ATTO 550, Cy®3, DyLight® 549, Rhodamine, TRITC
CF555-ddCTP
Figure 1. Absorption and emission spectra of CF555 goat anti-mouse antibody conjugate in PBS.

 

CF555 GAR, CF647 GAM, DAPI-icc2
Figure 2. MCF-7 cells stained with rabbit anti-COXIV and CF555 goat anti-rabbit (mitochondria, red) and mouse anti-ZO1 and CF647 goat anti-mouse (tight junctions, green). Mounted in EverBrite Mounting Medium with DAPI (nuclei, blue).
CF488A GAM min x rat, CF555 Mix-n-Stain, CF640R phalloidin-IHC
Figure 3. Frozen section of rat testis stained with mouse anti-tubulin and CF488A goat anti-mouse (min x rat) (microtubules, green), CF555 Mix-n-Stain labeled mouse anti-ZO1 (tight junctions, red) and CF640R phalloidin (actin filaments, cyan).