CF®555 is an orange-red fluorescent dye spectrally similar to Cy®3 and Alexa Fluor® 555. Like Alexa Fluor® 555, CF®555 is much brighter and more photostable than Cy®3. However, unlike Alexa Fluor® 555, which carries 4 negative charges, CF®555 achieves its superior performance without being excessively charged. Labeling antibodies with a highly charged dye can dramatically change the isoelectric point of the antibody, leading to increased background staining. CF®555 has been validated in multi-color STORM imaging (learn more about CF® Dyes for Super-Resolution Imaging). See CF®555 product lines below.
CF® 555 Technical Summary
- Abs/Em maxima: 555/565 nm
- Extinction coefficient: 150,000
- Molecular weight: ~901
- Excitation laser line: 532 nm, 555 nm, or 568 nm
- Direct replacement for: Alexa Fluor® 555, ATTO 550, Cy®3, DyLight® 549, Rhodamine, TRITC
CF is a registered trademark of Biotium, Inc. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of GE Healthcare.