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CF®555 Dye

Superior orange-red dye

Brighter than Cy®3
Highly specific conjugates
Validated in multicolor STORM

Figure 1. Normalized absorption and emission spectra of CF®555 goat anti-mouse conjugate in PBS.

CF®555 is an orange-red fluorescent dye spectrally similar to Cy®3 and Alexa Fluor® 555. Like Alexa Fluor® 555, CF®555 is much brighter and more photostable than Cy®3. However, unlike Alexa Fluor® 555, which carries 4 negative charges, CF®555 achieves its superior performance without being excessively charged. Labeling antibodies with a highly charged dye can dramatically change the isoelectric point of the antibody, leading to increased background staining. CF®555 has been validated in multi-color STORM imaging (learn more about CF® Dyes for Super-Resolution Imaging). See CF®555 product lines below.

CF® 555 Technical Summary

  • Abs/Em maxima: 555/565 nm
  • Extinction coefficient: 150,000
  • Molecular weight: ~901
  • Excitation laser line: 532 nm, 555 nm, or 568 nm
  • Direct replacement for: Alexa Fluor® 555, ATTO 550, Cy®3, DyLight® 549, Rhodamine, TRITC

Figure 2. Section of rat testis stained with mouse anti-tubulin and CF®488A goat anti-mouse (microtubules, green), followed by Mix-n-Stain™ CF®555 labeled mouse anti-ZO1 (tight junctions, red) and CF®640R phalloidin (actin filaments, cyan).
Figure 3. HeLa cells stained with rabbit anti-COXIV and CF®555 goat anti-rabbit (mitochondria, red), mouse anti-Golgin-97 and CF®488A goat anti-mouse (Golgi complex, green), and CF®640R phalloidin (actin filaments, cyan).
Figure 4. MCF7 cells stained with CF®555 monoclonal mouse anti-Cyclin B1 clone CCNB1/1098 (nuclei/nucleoli, red) and CF®488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).
 

CF is a registered trademark of Biotium, Inc. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of GE Healthcare.

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Bioconjugates

Lectins, proteins, nucleotides, toxins, and other probes conjugated to our bright & photostable CF® dyes and other labels.

Primary Antibodies

Growing collection of more than 1000 monoclonal antibodies validated in IHC and other applications. Available with bright and photostable CF® dyes, R-PE, APC, PerCP, HRP, AP, biotin, or purified. Affordable 100 uL trial sizes.

CF® Dye Labeled & Biotinylated Secondary Antibodies

Many host/target species and cross-absorption options, featuring a wide spectrum of our bright & photostable CF® dyes or biotin.

CF® Dye Single Label Conjugates for STORM

Secondary antibody conjugates of our STORM-compatible CF® dyes, with an average degree of labeling (DOL) of one, specifically designed for super-resolution imaging by STORM.

Mix-n-Stain™ Antibody Labeling Kits

Mix-n-Stain™ kits use revolutionary technology that dramatically simplifies antibody labeling and requires no purification. The labeling is covalent and stable. Available with your choice of 24 CF® dyes, biotin, FITC, fluorescent proteins, enzymes, or haptens.

CF® Dye Protein Labeling Kits

Everything you need to label and purify antibodies or other proteins. Choose from 19 bright & photostable CF® dyes or biotin.

Reactive CF® Dyes

Our bright, photostable, highly water soluble CF® dyes with a wide selection of colors and functional groups.

Additional conjugates or reactive dye forms may be added on request, contact techsupport@biotium.com to inquire.

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Faq

CF® Dyes

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

← FAQs
CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF® Dyes

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The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.

Category: CF® Dyes

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