EverBrite TrueBlack® Hardset Mounting Medium

TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF (Cat. No. 23007) is the original formulation. TrueBlack® Lipofuscin Autofluorescence Quencher, 30X in DMSO (Cat. No. 23011) is a newer, more concentrated formulation in DMSO, which is a less toxic solvent than DMF. The quencher stock solution formulation does not affect the performance in staining.

TrueBlack® Lipofuscin Autofluorescence Quencher and TrueBlack® Plus Lipofuscin Autofluorescence Quencher were not designed for colorimetric detection, but they will stain lipofuscin black and be visible in light microscopy.

TrueBlack® Lipofuscin Autofluorescence Quencher (Cat. No. 23007, 23011) and TrueBlack® Plus Lipofuscin Autofluorescence Quencher (Cat. No. 23014) are designed to reduce autofluorescence from lipofuscin in tissue samples such as mouse and human brains and retina. While TrueBlack® Lipofuscin Quenchers have been reported to reduce autofluorescence from other sources, such as collagen, elastin, red blood cells, and general background fluorescence, they are not as effective at quenching these types of autofluorescence as for lipofuscin autofluorescence. However, they may improve background from a variety of sources in different experimental systems.

TrueBlack® IF Background Suppressor System (Cat. No. 230120) is a buffer system designed for optimal blocking of non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections to eliminate non-specific staining for immunofluorescence (IF).

The TrueBlack® WB Blocking Buffer Kit (Cat. No. 23013) is a ready-to-use buffer system for blocking non-specific interactions of dye-labeled antibodies with proteins and the blotting membrane in fluorescence-based western blotting (WB).

EverBrite™ mounting medium does show high background fluorescence with violet excitation. The background is more prominent when imaged using epifluorescence using UV excitation and a DAPI filter cube, but is generally quite low in confocal imaging using 405 nm laser.

CoverGrip™ is designed to replace nail polish for permanent sealing of wet-mounted coverslips. It can be removed by soaking slides in buffer (see the product information sheet for details). CoverGrip™ is not designed to create a removable temporary seal for hybridization.

Yes, EverBrite™ mounting media are compatible with a wide range of dyes. Unlike VectaShield®, EverBrite™ won’t interfere with cyanine-based fluorophores like Alexa Fluor® 647, Cy®3, or Cy®5.

AlexaFluor is a registered trademark of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva; VectaShield is a registered trademark of Vector Laboratories.